Validation Data Gallery
Tested Applications
| Positive WB detected in | human saliva, tissue, pig pancreas tissue, rat pancreas tissue |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | BxPC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:600-1:2400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
Product Information
66133-1-Ig targets Pancreatic alpha-amylase in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat, pig samples.
| Tested Reactivity | human, rat, pig |
| Cited Reactivity | human, pig |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Pancreatic alpha-amylase fusion protein Ag8819 相同性解析による交差性が予測される生物種 |
| Full Name | amylase, alpha 2A (pancreatic) |
| Calculated molecular weight | 511 aa, 58 kDa |
| Observed molecular weight | 58 kDa |
| GenBank accession number | BC007060 |
| Gene Symbol | Amylase Alpha |
| Gene ID (NCBI) | 279 |
| RRID | AB_2881532 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P04746 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The complete human amylase gene contains five intact genes: two pancreatic genes (AMY2A and AMY2B) and three salivary genes (AMYIA, AMYIB, and AMYIC). AMY2A(Pancreatic alpha-amylase)is also named as PA and is a 56 kDa protein consisting of 496 amino acids in a single polypeptide chain, folded into three domains(PMID:10657258). It has a hydrolase activity acting on glycosyl bonds. AMY2A and AMY2B transcripts are present in the human pancreas but not in the parotid salivary gland, while AMY1 transcripts are present in the parotid gland but not in the pancreas(PMID:1692956).This antibody can recognize amylase alpha.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Pancreatic alpha-amylase antibody 66133-1-Ig | Download protocol |
| IHC protocol for Pancreatic alpha-amylase antibody 66133-1-Ig | Download protocol |
| IF protocol for Pancreatic alpha-amylase antibody 66133-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Am J Physiol Gastrointest Liver Physiol Is the intestinal mucous layer a NAtural Deep Eutectic Solvent (NADES) based digestion matrix | ||
Biochem Biophys Rep Multiple cystic sphere formation from PK-8 cells in three-dimensional culture | ||
J Proteome Res Proteomic Analysis of Differences in the Freezability of Porcine Sperm Identifies α-Amylase As a Key Protein |