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Species Reactivity

Species Reactivity

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Conjugate

Amylase Alpha Monoclonal antibody, PBS Only

Amylase Alpha Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat, pig

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F1G8

Cat no : 66133-1-PBS

Synonyms

AMY2A, Amylase Alpha, Amylase, alpha, amylase, alpha 2A (pancreatic), Pancreatic alpha amylase

Validation Data Gallery

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  • Western Blot (WB) analysis of human saliva using Amylase Alpha Monoclonal antibody (66133-1-Ig)

    WB analysis of human saliva using 66133-1-Ig (same clone as 66133-1-PBS)

    human saliva were subjected to SDS PAGE followed by western blot with 66133-1-Ig (Amylase, alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66133-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human saliva using Amylase Alpha Monoclonal antibody (66133-1-Ig)

    WB analysis of human saliva using 66133-1-Ig (same clone as 66133-1-PBS)

    0.1 μL human saliva lysate were subjected to SDS PAGE followed by western blot with 66133-1-Ig (Amylase, alpha antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66133-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig pancreas tissue using Amylase Alpha Monoclonal antibody (66133-1-Ig)

    WB analysis of pig pancreas using 66133-1-Ig (same clone as 66133-1-PBS)

    0.6 μg pig pancreas tissue lysate were subjected to SDS PAGE followed by western blot with 66133-1-Ig (Amylase, alpha antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66133-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat pancreas tissue using Amylase Alpha Monoclonal antibody (66133-1-Ig)

    WB analysis of rat pancreas using 66133-1-Ig (same clone as 66133-1-PBS)

    0.6 μg rat pancreas tissue lysate were subjected to SDS PAGE followed by western blot with 66133-1-Ig (Amylase, alpha antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66133-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Amylase Alpha Monoclonal antibody (66133-1-Ig)

    IHC staining of human pancreas cancer using 66133-1-Ig (same clone as 66133-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66133-1-Ig (Amylase, alpha antibody at dilution of 1:50(under 10x lens). This data was developed using the same antibody clone with 66133-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Amylase Alpha Monoclonal antibody (66133-1-Ig)

    IHC staining of human pancreas cancer using 66133-1-Ig (same clone as 66133-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66133-1-Ig (Amylase, alpha antibody at dilution of 1:50(under 40x lens). This data was developed using the same antibody clone with 66133-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of BxPC-3 cells using Amylase Alpha Monoclonal antibody (66133-1-Ig)

    IF Staining of BxPC-3 using 66133-1-Ig (same clone as 66133-1-PBS)

    Immunofluorescent analysis of BxPC-3 cells using 66133-1-Ig (Amylase, alpha antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 66133-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66133-1-PBS targets Amylase Alpha in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, rat, pig samples.

Tested Reactivity human, rat, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Amylase Alpha fusion protein Ag8819 相同性解析による交差性が予測される生物種
Full Name amylase, alpha 2A (pancreatic)
Calculated molecular weight 511 aa, 58 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC007060
Gene symbol AMY2A
Gene ID (NCBI) 279
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The complete human amylase gene contains five intact genes: two pancreatic genes (AMY2A and AMY2B) and three salivary genes (AMYIA, AMYIB, and AMYIC). AMY2A(Pancreatic alpha-amylase)is also named as PA and is a 56 kDa protein consisting of 496 amino acids in a single polypeptide chain, folded into three domains(PMID:10657258). It has a hydrolase activity acting on glycosyl bonds. AMY2A and AMY2B transcripts are present in the human pancreas but not in the parotid salivary gland, while AMY1 transcripts are present in the parotid gland but not in the pancreas(PMID:1692956).This antibody can recognize amylase alpha.