• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of human saliva using Pancreatic alpha-amylase Monoclonal antibody (66133-1-Ig)

    WB analysis of human saliva using 66133-1-Ig (same clone as 66133-1-PBS)

    human saliva were subjected to SDS PAGE followed by western blot with 66133-1-Ig (Amylase, alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66133-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human saliva using Pancreatic alpha-amylase Monoclonal antibody (66133-1-Ig)

    WB analysis of human saliva using 66133-1-Ig (same clone as 66133-1-PBS)

    0.1 μL human saliva lysate were subjected to SDS PAGE followed by western blot with 66133-1-Ig (Amylase, alpha antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66133-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig pancreas tissue using Pancreatic alpha-amylase Monoclonal antibody (66133-1-Ig)

    WB analysis of pig pancreas using 66133-1-Ig (same clone as 66133-1-PBS)

    0.6 μg pig pancreas tissue lysate were subjected to SDS PAGE followed by western blot with 66133-1-Ig (Amylase, alpha antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66133-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat pancreas tissue using Pancreatic alpha-amylase Monoclonal antibody (66133-1-Ig)

    WB analysis of rat pancreas using 66133-1-Ig (same clone as 66133-1-PBS)

    0.6 μg rat pancreas tissue lysate were subjected to SDS PAGE followed by western blot with 66133-1-Ig (Amylase, alpha antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66133-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Pancreatic alpha-amylase Monoclonal antibody (66133-1-Ig)

    IHC staining of human pancreas cancer using 66133-1-Ig (same clone as 66133-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66133-1-Ig (Amylase, alpha antibody at dilution of 1:50(under 10x lens). This data was developed using the same antibody clone with 66133-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Pancreatic alpha-amylase Monoclonal antibody (66133-1-Ig)

    IHC staining of human pancreas cancer using 66133-1-Ig (same clone as 66133-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66133-1-Ig (Amylase, alpha antibody at dilution of 1:50(under 40x lens). This data was developed using the same antibody clone with 66133-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of BxPC-3 cells using Pancreatic alpha-amylase Monoclonal antibody (66133-1-Ig)

    IF Staining of BxPC-3 using 66133-1-Ig (same clone as 66133-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed BxPC-3 cells using Amylase Alpha antibody (66133-1-Ig, Clone: 2F1G8 ) at dilution of 1:1200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66133-1-PBS in a different storage buffer formulation.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

Pancreatic alpha-amylase Monoclonal antibody, PBS Only

Pancreatic alpha-amylase Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66133-1-PBS
Clone No.2F1G8

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

AMY2A, Amylase Alpha, Amylase, alpha, AMY2B, AMY2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66133-1-PBS targets Pancreatic alpha-amylase in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat, pig samples.

Tested Reactivity human, rat, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Pancreatic alpha-amylase fusion protein Ag8819 相同性解析による交差性が予測される生物種
Full Name amylase, alpha 2A (pancreatic)
Calculated molecular weight 511 aa, 58 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC007060
Gene Symbol Amylase Alpha
Gene ID (NCBI) 279
RRIDAB_2881532
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP04746
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

The complete human amylase gene contains five intact genes: two pancreatic genes (AMY2A and AMY2B) and three salivary genes (AMYIA, AMYIB, and AMYIC). AMY2A(Pancreatic alpha-amylase)is also named as PA and is a 56 kDa protein consisting of 496 amino acids in a single polypeptide chain, folded into three domains(PMID:10657258). It has a hydrolase activity acting on glycosyl bonds. AMY2A and AMY2B transcripts are present in the human pancreas but not in the parotid salivary gland, while AMY1 transcripts are present in the parotid gland but not in the pancreas(PMID:1692956).This antibody can recognize amylase alpha.