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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Angiopoietin 1 Recombinant antibody (81990-4-RR)

    WB analysis using 81990-4-RR (same clone as 81990-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81990-4-RR (Angiopoietin 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81990-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Angiopoietin 1 Recombinant antibody (81990-4-RR)

    IHC staining of mouse heart using 81990-4-RR (same clone as 81990-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 81990-4-RR (Angiopoietin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81990-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using Angiopoietin 1 Recombinant antibody (81990-4-RR)

    FC experiment of HepG2 using 81990-4-RR (same clone as 81990-4-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Angiopoietin 1 Recombinant antibody (81990-4-RR, Clone:240764D10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 81990-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A431 cells using Angiopoietin 1 Recombinant antibody (81990-4-RR)

    FC experiment of A431 using 81990-4-RR (same clone as 81990-4-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Angiopoietin 1 Recombinant antibody (81990-4-RR, Clone:240764D10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 81990-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81990-4-RR

    Affinity and Kinetic Characterization of 81990-4-RR

    Biolayer interferometry (BLl) kinetic assays of 81990-4-RR against Human ANGPT1 were performed. The affinity constant is 6.37 nM.

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Angiopoietin 1 Recombinant antibody, PBS Only

Angiopoietin 1 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 81990-4-PBS
Clone No.240764D10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

ANGPT1, ANG-1, ANG1, ANG 1, AGPT

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81990-4-PBS targets Angiopoietin 1 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Angiopoietin 1 fusion protein Ag18829 相同性解析による交差性が予測される生物種
Full Name angiopoietin 1
Calculated molecular weight 498 aa, 58 kDa
Observed molecular weight70 kDa
GenBank accession numberBC152411
Gene Symbol Angiopoietin 1
Gene ID (NCBI) 284
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ15389
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Angiopoietin 1 is a 70-kDa secreted glycoprotein generated from vascular mural cells, pericytes, and certain other cells. Angiopoietin 1 participates in vascular differentiation through angiogenesis, which is the process of the growth and remodelling of existing vessels. Angiopoietin 1 is also involved in the maintenance and turnover of blood vessels in mature animals. Angiopoietin 1 binds to and activates the TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation, playing an important role in the regulation of angiogenesis. Overexpression of Angiopoietin 1 has been proven to occur in malignant glioblastoma, neuroblastoma, non-small cell lung cancer, and other tumors.