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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Angiopoietin 1 Recombinant antibody (81990-4-RR)

    WB analysis using 81990-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81990-4-RR (Angiopoietin 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Angiopoietin 1 Recombinant antibody (81990-4-RR)

    IHC staining of mouse heart using 81990-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 81990-4-RR (Angiopoietin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of HepG2 cells using Angiopoietin 1 Recombinant antibody (81990-4-RR)

    FC experiment of HepG2 using 81990-4-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Angiopoietin 1 Recombinant antibody (81990-4-RR, Clone:240764D10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of A431 cells using Angiopoietin 1 Recombinant antibody (81990-4-RR)

    FC experiment of A431 using 81990-4-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Angiopoietin 1 Recombinant antibody (81990-4-RR, Clone:240764D10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 81990-4-RR

    Affinity and Kinetic Characterization of 81990-4-RR

    Biolayer interferometry (BLl) kinetic assays of 81990-4-RR against Human ANGPT1 were performed. The affinity constant is 6.37 nM.

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Angiopoietin 1 Recombinant antibody

Angiopoietin 1 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 81990-4-RR
Clone No.240764D10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

ANGPT1, ANG-1, ANG1, ANG 1, AGPT

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, HeLa cells, U-87 MG cells
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHepG2 cells, A431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81990-4-RR targets Angiopoietin 1 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Angiopoietin 1 fusion protein Ag18829 相同性解析による交差性が予測される生物種
Full Name angiopoietin 1
Calculated molecular weight 498 aa, 58 kDa
Observed molecular weight70 kDa
GenBank accession numberBC152411
Gene Symbol Angiopoietin 1
Gene ID (NCBI) 284
RRIDAB_3670511
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ15389
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Angiopoietin 1 is a 70-kDa secreted glycoprotein generated from vascular mural cells, pericytes, and certain other cells. Angiopoietin 1 participates in vascular differentiation through angiogenesis, which is the process of the growth and remodelling of existing vessels. Angiopoietin 1 is also involved in the maintenance and turnover of blood vessels in mature animals. Angiopoietin 1 binds to and activates the TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation, playing an important role in the regulation of angiogenesis. Overexpression of Angiopoietin 1 has been proven to occur in malignant glioblastoma, neuroblastoma, non-small cell lung cancer, and other tumors.

Protocols

Product Specific Protocols
WB protocol for Angiopoietin 1 antibody 81990-4-RRDownload protocol
IHC protocol for Angiopoietin 1 antibody 81990-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols