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Validation Data Gallery

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  • Western Blot (WB) analysis of BxPC-3 cells using ANKZF1 Polyclonal antibody (20447-1-AP)

    WB analysis of BxPC-3 using 20447-1-AP (same clone as 20447-1-PBS)

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 20447-1-AP (ANKZF1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20447-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using ANKZF1 Polyclonal antibody (20447-1-AP)

    WB analysis of MCF-7 using 20447-1-AP (same clone as 20447-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 20447-1-AP (ANKZF1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20447-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ANKZF1 Polyclonal antibody (20447-1-AP)

    WB analysis of HeLa using 20447-1-AP (same clone as 20447-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 20447-1-AP (ANKZF1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20447-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using ANKZF1 Polyclonal antibody (20447-1-AP)

    WB analysis of HepG2 using 20447-1-AP (same clone as 20447-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 20447-1-AP (ANKZF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20447-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ANKZF1 Polyclonal antibody (20447-1-AP)

    WB analysis of HeLa using 20447-1-AP (same clone as 20447-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 20447-1-AP (ANKZF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20447-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using ANKZF1 Polyclonal antibody (20447-1-AP)

    WB analysis of COLO 320 using 20447-1-AP (same clone as 20447-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 20447-1-AP (ANKZF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20447-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using ANKZF1 Polyclonal antibody (20447-1-AP)

    WB analysis of MCF-7 using 20447-1-AP (same clone as 20447-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 20447-1-AP (ANKZF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20447-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using ANKZF1 Polyclonal antibody (20447-1-AP)

    IHC staining of human liver using 20447-1-AP (same clone as 20447-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20447-1-AP (ANKZF1 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 20447-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using ANKZF1 Polyclonal antibody (20447-1-AP)

    IHC staining of human liver using 20447-1-AP (same clone as 20447-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20447-1-AP (ANKZF1 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 20447-1-PBS in a different storage buffer formulation.

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ANKZF1 Polyclonal antibody, PBS Only

ANKZF1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 20447-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20447-1-PBS targets ANKZF1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ANKZF1 fusion protein Ag14285 相同性解析による交差性が予測される生物種
Full Name ankyrin repeat and zinc finger domain containing 1
Calculated molecular weight 726 aa, 81 kDa
Observed molecular weight 81 kDa
GenBank accession numberBC000238
Gene Symbol ANKZF1
Gene ID (NCBI) 55139
RRIDAB_2878688
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H8Y5
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.