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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ANO3 Polyclonal antibody (19489-1-AP)

    WB analysis using 19489-1-AP (same clone as 19489-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 19489-1-AP (ANO3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19489-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse skin tissue using ANO3 Polyclonal antibody (19489-1-AP)

    WB analysis of mouse skin using 19489-1-AP (same clone as 19489-1-PBS)

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 19489-1-AP (ANO3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19489-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse ovary tissue using ANO3 Polyclonal antibody (19489-1-AP)

    WB analysis of mouse ovary using 19489-1-AP (same clone as 19489-1-PBS)

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 19489-1-AP (ANO3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19489-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using ANO3 Polyclonal antibody (19489-1-AP)

    IP experiment of mouse testis using 19489-1-AP (same clone as 19489-1-PBS)

    IP result of anti-ANO3 (IP:19489-1-AP, 4ug; Detection:19489-1-AP 1:1000) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 19489-1-PBS in a different storage buffer formulation.

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ANO3 Polyclonal antibody, PBS Only

ANO3 Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 19489-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, Indirect ELISA

Synonyms

Transmembrane protein 16C, TMEM16C, GENX-3947, C11orf25, Anoctamin-3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19489-1-PBS targets ANO3 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name anoctamin 3
Calculated molecular weight 115 kDa
Observed molecular weight 100-115 kDa
GenBank accession numberNM_031418
Gene Symbol ANO3
Gene ID (NCBI) 63982
RRIDAB_10638489
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BYT9
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

ANO3, also named as C11orf25, TMEM16C and GENX-3947, belongs to the anoctamin family. It may act as a calcium-activated chloride channel.