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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated ANP32A Monoclonal antibody

ANP32A Monoclonal Antibody for WB, IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2D8C5

Cat no : CL594-67687

Synonyms

Leucine-rich acidic nuclear protein, LANP, I1PP2A, C15orf1, Acidic leucine-rich nuclear phosphoprotein 32 family member A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CoraLite®594-conjugated ANP32A Monoclonal antibody (CL594-67687)

    WB analysis using CL594-67687

    Various lysates were subjected to SDS PAGE followed by western blot with CL594-67687 (ANP32A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CoraLite®594-conjugated ANP32A Monoclonal antibody (CL594-67687)

    IF Staining of A431 using CL594-67687

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite®594 ANP32A antibody (CL594-67687, Clone: 2D8C5 ) at dilution of 1:200, CL488-Phalloidin (green).

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Tested Applications

Positive WB detected inJurkat cells, Raji cells, HeLa cells
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67687 targets ANP32A in WB, IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ANP32A fusion protein Ag8546 相同性解析による交差性が予測される生物種
Full Name acidic (leucine-rich) nuclear phosphoprotein 32 family, member A
Calculated molecular weight 249 aa, 29 kDa
Observed molecular weight29 kDa
GenBank accession numberBC007200
Gene symbol ANP32A
Gene ID (NCBI) 8125
RRIDAB_2920172
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ANP32A, an acidic leucine-rich nuclear phosphoprotein, is a member of the inhibitor of the histone acetyltransferase (INHAT) complex and preferentially binds to unmodified histone H3 tails in vitro. ANP32A is expressed in normal tissues as well as in pancreatic, breast and prostate cancer and other malignant tumors. ANP32A plays an important role in cell proliferation, apoptosis, transcriptional regulation, signal transduction and other processes.

Protocols

Product Specific Protocols
WB protocol for CL594 ANP32A antibody CL594-67687Download protocol
IF protocol for CL594 ANP32A antibody CL594-67687Download protocol
Standard Protocols
Click here to view our Standard Protocols