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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated ANP32B Monoclonal antibody (CL594-66160)

    IF Staining of HepG2 using CL594-66160

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 ANP32B antibody (CL594-66160, Clone: 5A1F12 ) at dilution of 1:50.

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  • KD/KO Validated

CoraLite®594-conjugated ANP32B Monoclonal antibody

ANP32B Monoclonal Antibody for IF/ICC
Cat No. CL594-66160
Clone No.5A1F12

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

ANP32B, APRIL, PHAPI2, Silver stainable protein SSP29, SSP29

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66160 targets ANP32B in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ANP32B fusion protein Ag1274 相同性解析による交差性が予測される生物種
Full Name acidic (leucine-rich) nuclear phosphoprotein 32 family, member B
Calculated molecular weight 29 kDa
GenBank accession numberBC013003
Gene Symbol ANP32B
Gene ID (NCBI) 10541
RRIDAB_2919955
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ92688
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ANP32B is a member of the highly conserved acidic leucine-rich nuclear phosphoprotein 32 (ANP32) family. The ANP32 proteins have been implicated in a broad array of physiological processes, including cell differentiation, apoptotic cell death, and cell proliferation. ANP32B was identified as a novel direct substrate for caspase-3 and acted as a negative regulator for leukemic cell apoptosis. ANP32B controls the expression of the dendritic cell maturation factor CD83 by regulating the transport of its mRNA to the cytoplasm. It also modulates the activity of the transcription factor Kruppel-like factor 5 (KLF5).

Protocols

Product Specific Protocols
IF protocol for CL594 ANP32B antibody CL594-66160Download protocol
Standard Protocols
Click here to view our Standard Protocols