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Annexin A2 抗体 46 文献

Rabbit Polyclonal| カタログ番号: 11256-1-AP

注目製品 KD/KO 検証済

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Related Primary Antibodies

適用アプリケーション

アプリケーション:
WB, RIP, IP, IHC, IF
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Images See all

Annexin A2 Antibody WB HeLa cells 11256-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 11256-1-AP (Annexin A2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Annexin A2 Antibody WB HEK-293 cells 11256-1-AP

WB result of Annexin A2 (11256-1-AP, 1:1000) with si-Control and si-Annexin A2 transfected HEK293 cells.

Annexin A2 Antibody IHC human liver tissue 11256-1-AP

Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11256-1-AP (Annexin A2 Antibody) at dilution of 1:200 (under 10x lens).

Annexin A2 Antibody IHC human liver tissue 11256-1-AP

Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11256-1-AP (Annexin A2 Antibody) at dilution of 1:200 (under 40x lens).

Annexin A2 Antibody IF T-47D cells 11256-1-AP

Immunofluorescent analysis of (4% PFA) fixed T-47D cells using Annexin A2 antibody (11256-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Annexin A2 Antibody IP HeLa cells 11256-1-AP

IP result of anti-Annexin A2 (IP:11256-1-AP, 4ug; Detection:11256-1-AP 1:3000) with HeLa cells lysate 2640 ug.

種特異性:
human, mouse, rat, chicken, monkey
WB ポジティブ:
HeLa cells, HEK-293 cells, HepG2 cells, NIH/3T3 cells, mouse placenta tissue, rat uterus tissue, nouse kidney tissue
IP ポジティブ:
HeLa cells
IHC ポジティブ:
human liver tissue, human breast cancer tissue, human prostate cancer tissue, human oesophagus cancer tissue, human colon cancer tissue, human hepatocirrhosis tissue
IF ポジティブ:
T-47D cells, HeLa cells, MCF-7 cells
推奨希釈倍率:
WB : 1:1000-1:6000
IP : 0.5-4.0 ug for IP and 1:1000-1:6000 for WB
IHC : 1:50-1:500
IF : 1:200-1:800

Protocols

Product-specific protocols

Standard Protocols

製品情報

由来:
Rabbit
精製方法:
Antigen affinity purification
アイソタイプ:
IgG
バッファー/保存方法:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

ターゲット情報

免疫原:
Annexin A2 fusion protein Ag1777
正式名:
annexin A2
計算分子量:
339 aa, 39 kDa
検出分子量:
35-40 kDa
GenBank accession number:
BC016774
Gene ID (NCBI):
302
遺伝子シンボル:
ANXA2
別名:
Annexin 2, annexin A2, Annexin II, ANX2, ANX2L4, ANXA2, CAL1H, Calpactin 1 heavy chain, Calpactin I heavy chain, Chromobindin 8, LIP2, Lipocortin II, LPC2, LPC2D, P36, PAP IV, Protein I

背景説明

Annexin A2 (ANXA2), also named Annexin-2 or Lipocortin II, is a Ca2+ binding protein that is up-regulated in virally transformed cell lines and human tumors. This calcium-regulated membrane-binding protein, whose affinity for calcium is greatly enhanced by anionic phospholipids, may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Heat-stress response may also involve Annexin-2.


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