Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 11256-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11256-1-AP (Annexin A2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis using 11256-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11256-1-AP (Annexin A2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 11256-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11256-1-AP (Annexin A2 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.
WB analysis using 11256-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11256-1-AP (Annexin A2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 11256-1-AP
WB result of Annexin A2 (11256-1-AP, 1:1000) with si-Control and si-Annexin A2 transfected HEK293 cells.
WB analysis of THP-1 using 11256-1-AP
WB result of Annexin A2 antibody (11256-1-AP; 1:20000; room temperature for 1.5 hours) with wild-type and sg-Annexin A2 transfected THP-1 cells.
WB analysis of rat uterus using 11256-1-AP
rat uterus tissue were subjected to SDS PAGE followed by western blot with 11256-1-AP (Annexin A2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse placenta using 11256-1-AP
mouse placenta tissue were subjected to SDS PAGE followed by western blot with 11256-1-AP (Annexin A2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 11256-1-AP
IP result of anti-Annexin A2 (IP:11256-1-AP, 4ug; Detection:11256-1-AP 1:30000) with HeLa cells lysate 1600 ug.
IHC staining of human liver using 11256-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11256-1-AP (Annexin A2 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human liver using 11256-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11256-1-AP (Annexin A2 Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of mouse liver using 11256-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 11256-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse placenta using 11256-1-AP
Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse placenta using 11256-1-AP
Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 11256-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 11256-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse placenta using 11256-1-AP
Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 11256-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human bowen disease using 11256-1-AP
Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human bowen disease using 11256-1-AP
Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human hepatocirrhosis using 11256-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human hepatocirrhosis using 11256-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 11256-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 11256-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 11256-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 11256-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human oesophagus cancer using 11256-1-AP
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human oesophagus cancer using 11256-1-AP
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 11256-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 11256-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 11256-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 11256-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of MCF-7 using 11256-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Annexin A2 antibody (11256-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
IF Staining of HeLa using 11256-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Annexin A2 antibody (11256-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).
IF Staining of MCF-7 using 11256-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 11256-1-AP (Annexin A2 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of MCF-7 using 11256-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using Annexin A2 antibody (11256-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 11256-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Annexin A2 antibody (11256-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 11256-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Annexin A2 antibody (11256-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of K-562 using 11256-1-AP
1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human Annexin A2 (11256-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
FC experiment of A549 using 11256-1-AP
1x10^6 A549 cells were intracellularly stained with 0.25 ug Annexin A2 Polyclonal antibody (11256-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO Validated

Annexin A2 Polyclonal antibody

Annexin A2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 11256-1-AP

Publications(76)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA

Synonyms

ANXA2, Annexin 2, Annexin II, Annexin-2, ANX2

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (76)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, mouse placenta tissue, THP-1 cells, NIH/3T3 cells, mouse kidney tissue, rat kidney tissue, HepG2 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	mouse placenta tissue, human bowen disease tissue, human breast cancer tissue, human hepatocirrhosis tissue, human liver tissue, human lung cancer tissue, human oesophagus cancer tissue, human prostate cancer tissue, mouse kidney tissue, mouse liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, MCF-7 cells
Positive FC (Intra) detected in	K-562 cells, A549 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 10 publications below
WB	See 58 publications below
IHC	See 14 publications below
IF	See 19 publications below
IP	See 11 publications below
ELISA	See 1 publications below
CoIP	See 1 publications below
RIP	See 2 publications below

Product Information

11256-1-AP targets Annexin A2 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, monkey, chicken, zebrafish, bovine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Annexin A2 fusion protein Ag1777 相同性解析による交差性が予測される生物種
Full Name	annexin A2
Calculated molecular weight	339 aa, 39 kDa
Observed molecular weight	35-40 kDa
GenBank accession number	BC016774
Gene Symbol	Annexin A2
Gene ID (NCBI)	302
ENSEMBL Gene ID	ENSG00000182718
RRID	AB_2057311
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P07355
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Annexin A2 (ANXA2), also named Annexin-2 or Lipocortin II, is a Ca2+ binding protein that is up-regulated in virally transformed cell lines and human tumors. This calcium-regulated membrane-binding protein, whose affinity for calcium is greatly enhanced by anionic phospholipids, may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Heat-stress response may also involve Annexin-2.

Protocols

Product Specific Protocols
WB protocol for Annexin A2 antibody 11256-1-AP	Download protocol
IHC protocol for Annexin A2 antibody 11256-1-AP	Download protocol
IF protocol for Annexin A2 antibody 11256-1-AP	Download protocol
IP protocol for Annexin A2 antibody 11256-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human		Nat Immunol NKILA lncRNA promotes tumor immune evasion by sensitizing T cells to activation-induced cell death. Authors - Di Huang View Article
mouse	WB	Adv Sci (Weinh) Isolation and Comprehensive Analysis of Cochlear Tissue-Derived Small Extracellular Vesicles Authors - Pei Jiang View Article
human	WB	Sci Adv p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi. Authors - Xiaochao Tan View Article
human	WB	Nat Chem Biol YAP-dependent proliferation by a small molecule targeting annexin A2. Authors - Sophia Z Shalhout KD Validated View Article
human	WB	Nat Commun PMID: 31792210 Authors - Sunwang Xu View Article
mouse	WB	Nucleic Acids Res Short DNA/RNA heteroduplex oligonucleotide interacting proteins are key regulators of target gene silencing. Authors - Ken Asada View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 11256-1-AP

WB analysis using 11256-1-AP

WB analysis using 11256-1-AP

WB analysis using 11256-1-AP

WB analysis of HEK-293 using 11256-1-AP

WB analysis of THP-1 using 11256-1-AP

WB analysis of rat uterus using 11256-1-AP

WB analysis of mouse placenta using 11256-1-AP

IP experiment of HeLa using 11256-1-AP

IHC staining of human liver using 11256-1-AP

IHC staining of human liver using 11256-1-AP

IHC staining of mouse liver using 11256-1-AP

IHC staining of mouse liver using 11256-1-AP

IHC staining of mouse placenta using 11256-1-AP

IHC staining of mouse placenta using 11256-1-AP

IHC staining of mouse kidney using 11256-1-AP

IHC staining of mouse liver using 11256-1-AP

IHC staining of mouse placenta using 11256-1-AP

IHC staining of human lung cancer using 11256-1-AP

IHC staining of human bowen disease using 11256-1-AP

IHC staining of human bowen disease using 11256-1-AP

IHC staining of human hepatocirrhosis using 11256-1-AP

IHC staining of human hepatocirrhosis using 11256-1-AP

IHC staining of human breast cancer using 11256-1-AP

IHC staining of human breast cancer using 11256-1-AP

IHC staining of human prostate cancer using 11256-1-AP

IHC staining of human prostate cancer using 11256-1-AP

IHC staining of human oesophagus cancer using 11256-1-AP

IHC staining of human oesophagus cancer using 11256-1-AP

IHC staining of human colon cancer using 11256-1-AP

IHC staining of human colon cancer using 11256-1-AP

IHC staining of human breast cancer using 11256-1-AP

IHC staining of human breast cancer using 11256-1-AP

IF Staining of MCF-7 using 11256-1-AP

IF Staining of HeLa using 11256-1-AP

IF Staining of MCF-7 using 11256-1-AP

IF Staining of MCF-7 using 11256-1-AP

IF Staining of HeLa using 11256-1-AP

IF Staining of HeLa using 11256-1-AP

FC experiment of K-562 using 11256-1-AP

FC experiment of A549 using 11256-1-AP

Annexin A2 Polyclonal antibody

Annexin A2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

NKILA lncRNA promotes tumor immune evasion by sensitizing T cells to activation-induced cell death.

Isolation and Comprehensive Analysis of Cochlear Tissue-Derived Small Extracellular Vesicles

p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi.

YAP-dependent proliferation by a small molecule targeting annexin A2.

PMID: 31792210

Short DNA/RNA heteroduplex oligonucleotide interacting proteins are key regulators of target gene silencing.