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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Annexin A2 Polyclonal antibody

Annexin A2 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-11256

Synonyms

ANXA2, ANX2L4, ANX2, Annexin-2, Annexin II

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CoraLite® Plus 488-conjugated Annexin A2 Polyclona (CL488-11256)

    IF Staining of MCF-7 using CL488-11256

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 Annexin A2 antibody (CL488-11256) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated Annexin A2 Polyclona (CL488-11256)

    IF Staining of HeLa using CL488-11256

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 Annexin A2 antibody (CL488-11256) at dilution of 1:200.

  • Flow cytometry (FC) experiment of K-562 cells using CoraLite® Plus 488-conjugated Annexin A2 Polyclona (CL488-11256)

    FC experiment of K-562 using CL488-11256

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Annexin A2 (CL488-11256) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated Annexin A2 Polyclona (CL488-11256)

    FC experiment of HeLa using CL488-11256

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated Annexin A2 Polyclonal antibody (CL488-11256)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inMCF-7 cells, HeLa cells
Positive FC (Intra) detected inK-562 cells, HeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-11256 targets Annexin A2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Annexin A2 fusion protein Ag1777 相同性解析による交差性が予測される生物種
Full Name annexin A2
Calculated molecular weight 339 aa, 39 kDa
Observed molecular weight 35-40 kDa
GenBank accession numberBC016774
Gene symbol Annexin A2
Gene ID (NCBI) 302
RRIDAB_2919020
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Annexin A2 (ANXA2), also named Annexin-2 or Lipocortin II, is a Ca2+ binding protein that is up-regulated in virally transformed cell lines and human tumors. This calcium-regulated membrane-binding protein, whose affinity for calcium is greatly enhanced by anionic phospholipids, may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Heat-stress response may also involve Annexin-2.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Annexin A2 antibody CL488-11256Download protocol
FC protocol for CL Plus 488 Annexin A2 antibody CL488-11256Download protocol
Standard Protocols
Click here to view our Standard Protocols