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  • KD/KO Validated

Annexin A2 Monoclonal antibody

Annexin A2 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

3H1B11

Cat no : 60051-1-Ig

Synonyms

ANXA2, ANX2L4, ANX2, Annexin-2, Annexin II


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Tested Applications

Positive WB detected inK-562 cells, HEK-293 cells, A431 cells, A549 cells, HeLa cells, HepG2 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human lung cancer tissue, human skin cancer tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inT-47D cells, HeLa cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60051-1-Ig targets Annexin A2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Annexin A2 fusion protein Ag1777 相同性解析による交差性が予測される生物種
Full Name annexin A2
Calculated molecular weight 339 aa, 39 kDa
Observed molecular weight 35-38 kDa
GenBank accession numberBC016774
Gene symbol Annexin A2
Gene ID (NCBI) 302
RRIDAB_2057309
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Annexin A2 (ANXA2), also named Annexin-2 or Lipocortin II, is a Ca2+ binding protein that is up-regulated in virally transformed cell lines and human tumors. This calcium-regulated membrane-binding protein, whose affinity for calcium is greatly enhanced by anionic phospholipids, may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Heat-stress response may also involve Annexin-2.

Protocols

Product Specific Protocols
WB protocol for Annexin A2 antibody 60051-1-IgDownload protocol
IHC protocol for Annexin A2 antibody 60051-1-IgDownload protocol
IF protocol for Annexin A2 antibody 60051-1-IgDownload protocol
IP protocol for Annexin A2 antibody 60051-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,CoIP

Cell

Streptococcus anginosus promotes gastric inflammation, atrophy, and tumorigenesis in mice

Authors - Kaili Fu
humanIP

EBioMedicine

Compounds targeting YadC of uropathogenic Escherichia coli and its host receptor annexin A2 decrease bacterial colonization in bladder.

Authors - Xiao Li

Infect Immun

The Tick Protein Sialostatin L2 Binds to Annexin A2 and Inhibits NLRC4-Mediated Inflammasome Activation.

Authors - Xiaowei Wang
mouseWB

Cell Mol Life Sci

Upregulation of HDAC9 in hippocampal neurons mediates depression-like behaviours by inhibiting ANXA2 degradation

Authors - Yunjian Dai
human,mouseWB,IP,IF

iScience

Opportunistic pathogen Porphyromonas gingivalis targets the LC3B-ceramide complex and mediates lethal mitophagy resistance in oral tumors

Authors - Megan Sheridan
  • KD Validated
humanWB,IF

Cell Signal

The novel DNA methylation marker FIBIN suppresses non-small cell lung cancer metastasis by negatively regulating ANXA2

Authors - Mingyu Peng