Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 60051-1-Ig (same clone as 60051-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 60051-1-Ig (Annexin A2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.
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Various lysates were subjected to SDS PAGE followed by western blot with 60051-1-Ig (Annexin A2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 60051-1-Ig (same clone as 60051-1-PBS)
WB result of Annexin A2 antibody (60051-1-Ig, 1:20,000) with si-Control and si-Annexin A2 transfected HEK293 cells. This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.
WB analysis of THP-1 using 60051-1-Ig (same clone as 60051-1-PBS)
WB result of Annexin A2 antibody (60051-1-Ig; 1:10000; room temperature for 1.5 hours) with wild-type and sg-Annexin A2 transfected THP-1 cells. This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.
IP experiment of HeLa using 60051-1-Ig (same clone as 60051-1-PBS)
IP result of anti-Annexin A2 (IP:60051-1-Ig, 4ug; Detection:60051-1-Ig 1:1500) with HeLa cells lysate 2400ug. This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 60051-1-Ig (same clone as 60051-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60051-1-Ig (Annexin A2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 60051-1-Ig (same clone as 60051-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60051-1-Ig (Annexin A2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.
IHC staining of human gliomas using 60051-1-Ig (same clone as 60051-1-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas slide using 60051-1-Ig (ANXA2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.
IHC staining of human gliomas using 60051-1-Ig (same clone as 60051-1-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas slide using 60051-1-Ig (ANXA2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 60051-1-Ig (same clone as 60051-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60051-1-Ig (Annexin A2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 60051-1-Ig (same clone as 60051-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60051-1-Ig (Annexin A2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.
IHC staining of human skin cancer using 60051-1-Ig (same clone as 60051-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 60051-1-Ig (Annexin A2 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.
IHC staining of human skin cancer using 60051-1-Ig (same clone as 60051-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 60051-1-Ig (Annexin A2 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.
IF Staining of T-47D using 60051-1-Ig (same clone as 60051-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed T-47D cells using 60051-1-Ig (Annexin A2 antibody), at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.
IF Staining of MCF-7 using 60051-1-Ig (same clone as 60051-1-PBS)
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Annexin A2 antibody (60051-1-Ig, Clone: 3H1B11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 60051-1-Ig (same clone as 60051-1-PBS)
Immunofluorescent analysis of Hela cells, using ANXA2 antibody 66051-1-lg at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.
IF Staining of MCF-7 using 60051-1-Ig (same clone as 60051-1-PBS)
Immunofluorescent analysis of MCF-7 cells, using ANXA2 antibody 60051-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 60051-1-Ig (same clone as 60051-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 60051-1-Ig (Annexin A2 antibody), at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

Annexin A2 Monoclonal antibody, PBS Only

Annexin A2 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 60051-1-PBS

Clone No.3H1B11

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

ANXA2, ANX2L4, ANX2, Annexin-2, Annexin II

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60051-1-PBS targets Annexin A2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Immunogen	Annexin A2 fusion protein Ag1777 相同性解析による交差性が予測される生物種
Full Name	annexin A2
Calculated molecular weight	339 aa, 39 kDa
Observed molecular weight	35-38 kDa
GenBank accession number	BC016774
Gene Symbol	Annexin A2
Gene ID (NCBI)	302
ENSEMBL Gene ID	ENSG00000182718
RRID	AB_2057309
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P07355
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

Annexin A2 (ANXA2), also named Annexin-2 or Lipocortin II, is a Ca2+ binding protein that is up-regulated in virally transformed cell lines and human tumors. This calcium-regulated membrane-binding protein, whose affinity for calcium is greatly enhanced by anionic phospholipids, may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Heat-stress response may also involve Annexin-2.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 60051-1-Ig (same clone as 60051-1-PBS)

WB analysis of HEK-293 using 60051-1-Ig (same clone as 60051-1-PBS)

WB analysis of THP-1 using 60051-1-Ig (same clone as 60051-1-PBS)

IP experiment of HeLa using 60051-1-Ig (same clone as 60051-1-PBS)

IHC staining of human breast cancer using 60051-1-Ig (same clone as 60051-1-PBS)

IHC staining of human breast cancer using 60051-1-Ig (same clone as 60051-1-PBS)

IHC staining of human gliomas using 60051-1-Ig (same clone as 60051-1-PBS)