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Annexin A2 Monoclonal antibody, PBS Only

Name: Annexin A2 Monoclonal antibody, PBS Only
Brand: Proteintech
SKU: 60051-1-PBS

Annexin A2 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3H1B11

Review Product

Cat no : 60051-1-PBS

Synonyms

ANXA2, ANX2L4, ANX2, Annexin-2, Annexin II

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 60051-1-Ig (same clone as 60051-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 60051-1-Ig (Annexin A2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.
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Various lysates were subjected to SDS PAGE followed by western blot with 60051-1-Ig (Annexin A2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 60051-1-Ig (same clone as 60051-1-PBS)
WB result of Annexin A2 antibody (60051-1-Ig, 1:20,000) with si-Control and si-Annexin A2 transfected HEK293 cells. This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.
IP experiment of HeLa using 60051-1-Ig (same clone as 60051-1-PBS)
IP result of anti-Annexin A2 (IP:60051-1-Ig, 4ug; Detection:60051-1-Ig 1:1500) with HeLa cells lysate 2400ug. This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 60051-1-Ig (same clone as 60051-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60051-1-Ig (Annexin A2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 60051-1-Ig (same clone as 60051-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60051-1-Ig (Annexin A2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 60051-1-Ig (same clone as 60051-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60051-1-Ig (Annexin A2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 60051-1-Ig (same clone as 60051-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60051-1-Ig (Annexin A2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.
IHC staining of human skin cancer using 60051-1-Ig (same clone as 60051-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 60051-1-Ig (Annexin A2 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.
IHC staining of human skin cancer using 60051-1-Ig (same clone as 60051-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 60051-1-Ig (Annexin A2 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.
IHC staining of human gliomas using 60051-1-Ig (same clone as 60051-1-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas slide using 60051-1-Ig (ANXA2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.
IHC staining of human gliomas using 60051-1-Ig (same clone as 60051-1-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas slide using 60051-1-Ig (ANXA2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.
IF Staining of T-47D using 60051-1-Ig (same clone as 60051-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed T-47D cells using 60051-1-Ig (Annexin A2 antibody), at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.
IF Staining of MCF-7 using 60051-1-Ig (same clone as 60051-1-PBS)
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Annexin A2 antibody (60051-1-Ig, Clone: 3H1B11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 60051-1-Ig (same clone as 60051-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 60051-1-Ig (Annexin A2 antibody), at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 60051-1-Ig (same clone as 60051-1-PBS)
Immunofluorescent analysis of Hela cells, using ANXA2 antibody 66051-1-lg at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.
IF Staining of MCF-7 using 60051-1-Ig (same clone as 60051-1-PBS)
Immunofluorescent analysis of MCF-7 cells, using ANXA2 antibody 60051-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.

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Cat No. 60051-1-PBS

Size/Concentration

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Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60051-1-PBS targets Annexin A2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Immunogen	Annexin A2 fusion protein Ag1777 相同性解析による交差性が予測される生物種
Full Name	annexin A2
Calculated molecular weight	339 aa, 39 kDa
Observed molecular weight	35-38 kDa
GenBank accession number	BC016774
Gene symbol	Annexin A2
Gene ID (NCBI)	302
RRID	AB_2057309
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

Annexin A2 (ANXA2), also named Annexin-2 or Lipocortin II, is a Ca2+ binding protein that is up-regulated in virally transformed cell lines and human tumors. This calcium-regulated membrane-binding protein, whose affinity for calcium is greatly enhanced by anionic phospholipids, may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Heat-stress response may also involve Annexin-2.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Annexin A2 Monoclonal antibody, PBS Only

Annexin A2 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Validation Data Gallery

WB analysis using 60051-1-Ig (same clone as 60051-1-PBS)

WB analysis of HEK-293 using 60051-1-Ig (same clone as 60051-1-PBS)

IP experiment of HeLa using 60051-1-Ig (same clone as 60051-1-PBS)

IHC staining of human breast cancer using 60051-1-Ig (same clone as 60051-1-PBS)

IHC staining of human breast cancer using 60051-1-Ig (same clone as 60051-1-PBS)

IHC staining of human lung cancer using 60051-1-Ig (same clone as 60051-1-PBS)

IHC staining of human lung cancer using 60051-1-Ig (same clone as 60051-1-PBS)

IHC staining of human skin cancer using 60051-1-Ig (same clone as 60051-1-PBS)

IHC staining of human skin cancer using 60051-1-Ig (same clone as 60051-1-PBS)

IHC staining of human gliomas using 60051-1-Ig (same clone as 60051-1-PBS)