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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human colon tissue using Annexin V Recombinant antibody (83929-2-RR)

    IHC staining of human colon using 83929-2-RR (same clone as 83929-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 83929-2-RR (ANXA5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83929-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Annexin V Recombinant antibody (83929-2-RR)

    IHC staining of human colon using 83929-2-RR (same clone as 83929-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 83929-2-RR (ANXA5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83929-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Annexin V Recombinant antibody (83929-2-RR)

    IF Staining of HeLa using 83929-2-RR (same clone as 83929-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ANXA5 antibody (83929-2-RR, Clone: 240621B10 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83929-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of PC-3 cells using Annexin V Recombinant antibody (83929-2-RR)

    FC experiment of PC-3 using 83929-2-RR (same clone as 83929-2-PBS)

    1x10^6 PC-3 cells were intracellularly stained with 0.25 ug Anxa5 Recombinant Antibody (83929-2-RR, Clone:240621B10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83929-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using Annexin V Recombinant antibody (83929-2-RR)

    FC experiment of HeLa using 83929-2-RR (same clone as 83929-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anxa5 Recombinant Antibody (83929-2-RR, Clone:240621B10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83929-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83929-2-RR

    Affinity and Kinetic Characterization of 83929-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83929-2-RR against Human ANXA5 were performed. The affinity constant is 18.2 nM.

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Annexin V Recombinant antibody, PBS Only

Annexin V Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83929-2-PBS
Clone No.240621B10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), ELISA

Synonyms

Annexin A5, ANXA5, 240621B10, Anchorin CII, Annexin 5

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83929-2-PBS targets Annexin V in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Annexin V fusion protein Ag0741 相同性解析による交差性が予測される生物種
Full Name annexin A5
Calculated molecular weight 36 kDa
Observed molecular weight 32-36 kDa
GenBank accession numberBC001429
Gene Symbol Annexin V
Gene ID (NCBI) 308
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP08758
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Annexin A5 (ANXA5), is a member of the structurally related family of annexin proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII.