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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ANXA5 Recombinant antibody

ANXA5 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240621B10

Cat no : 83929-2-RR

Synonyms

Annexin A5, Annexin-5, Annexin V, Annexin 5, Anchorin CII

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ANXA5 Recombinant antibody (83929-2-RR)

    WB analysis using 83929-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83929-2-RR (ANXA5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ANXA5 Recombinant antibody (83929-2-RR)

    IF Staining of HeLa using 83929-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ANXA5 antibody (83929-2-RR, Clone: 240621B10 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of PC-3 cells using ANXA5 Recombinant antibody (83929-2-RR)

    FC experiment of PC-3 using 83929-2-RR

    1x10^6 PC-3 cells were intracellularly stained with 0.25 ug Anxa5 Recombinant Antibody (83929-2-RR, Clone:240621B10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of HeLa cells using ANXA5 Recombinant antibody (83929-2-RR)

    FC experiment of HeLa using 83929-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anxa5 Recombinant Antibody (83929-2-RR, Clone:240621B10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83929-2-RR

    Affinity and Kinetic Characterization of 83929-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83929-2-RR against Human ANXA5 were performed. The affinity constant is 18.2 nM.

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Tested Applications

Positive WB detected inC6 cells, HeLa cells, K-562 cells, NIH/3T3 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inPC-3 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83929-2-RR is ANXA5 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ANXA5 fusion protein Ag0741 相同性解析による交差性が予測される生物種
Full Name annexin A5
Calculated molecular weight 36 kDa
Observed molecular weight 32-36 kDa
GenBank accession numberBC001429
Gene symbol Annexin V
Gene ID (NCBI) 308
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Annexin A5 (ANXA5), is a member of the structurally related family of annexin proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII.

Protocols

Product Specific Protocols
WB protocol for ANXA5 antibody 83929-2-RRDownload protocol
IF protocol for ANXA5 antibody 83929-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols