ANXA5 Recombinant antibody

ANXA5 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240621B10

Cat no : 83929-2-RR

Synonyms

Annexin A5, Annexin-5, Annexin V, Annexin 5, Anchorin CII


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Tested Applications

Positive WB detected inC6 cells, HeLa cells, K-562 cells, NIH/3T3 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inPC-3 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83929-2-RR is ANXA5 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ANXA5 fusion protein Ag0741 相同性解析による交差性が予測される生物種
Full Name annexin A5
Calculated molecular weight 36 kDa
Observed molecular weight 32-36 kDa
GenBank accession numberBC001429
Gene symbol Annexin V
Gene ID (NCBI) 308
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Annexin A5 (ANXA5), is a member of the structurally related family of annexin proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII.

Protocols

Product Specific Protocols
WB protocol for ANXA5 antibody 83929-2-RRDownload protocol
IF protocol for ANXA5 antibody 83929-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols