ANXA5 Recombinant antibody
ANXA5 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IF/ICC, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
240621B10
Cat no : 83929-2-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | C6 cells, HeLa cells, K-562 cells, NIH/3T3 cells |
Positive IF/ICC detected in | HeLa cells |
Positive FC (Intra) detected in | PC-3 cells, HeLa cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
The immunogen of 83929-2-RR is ANXA5 Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | ANXA5 fusion protein Ag0741 相同性解析による交差性が予測される生物種 |
Full Name | annexin A5 |
Calculated molecular weight | 36 kDa |
Observed molecular weight | 32-36 kDa |
GenBank accession number | BC001429 |
Gene symbol | Annexin V |
Gene ID (NCBI) | 308 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Annexin A5 (ANXA5), is a member of the structurally related family of annexin proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII.
Protocols
Product Specific Protocols | |
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WB protocol for ANXA5 antibody 83929-2-RR | Download protocol |
IF protocol for ANXA5 antibody 83929-2-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |