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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated AOC3 Monoclonal antibody

AOC3 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2A6G3

Cat no : CL488-66834

Synonyms

AOC3, Copper amine oxidase, HPAO, Membrane primary amine oxidase, SSAO, VAP 1, VAP1, Vascular adhesion protein 1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using CoraLite® Plus 488-conjugated AOC3 Monoclonal anti (CL488-66834)

    IF Staining of human liver cancer using CL488-66834

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite® Plus 488 AOC3 antibody (CL488-66834, Clone: 2A6G3 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using CoraLite® Plus 488-conjugated AOC3 Monoclonal anti (CL488-66834)

    IF Staining of human liver cancer using CL488-66834

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite® Plus 488 AOC3 antibody (CL488-66834, Clone: 2A6G3 ) at dilution of 1:200.

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Tested Applications

Positive IF-P detected inhuman liver cancer tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66834 targets AOC3 in IF-P applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AOC3 fusion protein Ag5908 相同性解析による交差性が予測される生物種
Full Name amine oxidase, copper containing 3 (vascular adhesion protein 1)
Calculated molecular weight 85 kDa
Observed molecular weight 85-90 kDa
GenBank accession numberBC050549
Gene symbol AOC3
Gene ID (NCBI) 8639
RRIDAB_2919378
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AOC3(membrane primary amine oxidase) is also name as copper amine oxidase, HPAO, SSAO, VAP1 and belongs to the copper/topaquinone oxidase family. It is expressed on the surface of endothelial cells and is involved in leukocyte trafficking between blood and tissues under physiologic and pathologic conditions (PMID:17947691). AOC3 is highly expressed in adipocytes and smooth muscle cells and it can be N- and O-glycosylated (PMID:16046623). AOC3 has molecular mass of 70-90kD, and can exsit as a homodimer(165-185kD) and trimer(240~260kD) (PMID:10595925, 8625974).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 AOC3 antibody CL488-66834Download protocol
Standard Protocols
Click here to view our Standard Protocols