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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using AP1M1 Recombinant antibody (83613-6-RR)

    WB analysis of HEK-293 using 83613-6-RR (same clone as 83613-6-PBS)

    WB result of AP1M1 antibody (83613-6-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AP1M1 transfected HEK-293 cells. This data was developed using the same antibody clone with 83613-6-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using AP1M1 Recombinant antibody (83613-6-RR)

    WB analysis using 83613-6-RR (same clone as 83613-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83613-6-RR (AP1M1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83613-6-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using AP1M1 Recombinant antibody (83613-6-RR)

    WB analysis using 83613-6-RR (same clone as 83613-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83613-6-RR (AP1M1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83613-6-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skin tissue using AP1M1 Recombinant antibody (83613-6-RR)

    IHC staining of mouse skin using 83613-6-RR (same clone as 83613-6-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 83613-6-RR (AP1M1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83613-6-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using AP1M1 Recombinant antibody (83613-6-RR)

    FC experiment of HeLa using 83613-6-RR (same clone as 83613-6-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug AP1M1 Recombinant antibody (83613-6-RR, Clone:240553H3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83613-6-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U-251 cells using AP1M1 Recombinant antibody (83613-6-RR)

    FC experiment of U-251 using 83613-6-RR (same clone as 83613-6-PBS)

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug AP1M1 Recombinant antibody (83613-6-RR, Clone:240553H3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83613-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83613-6-RR

    Affinity and Kinetic Characterization of 83613-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83613-6-RR against Human AP1M1 were performed. The affinity constant is below 1 pM.

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  • Featured Product
  • KD/KO Validated

AP1M1 Recombinant antibody, PBS Only

AP1M1 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 83613-6-PBS
Clone No.240553H3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

AP-1 complex subunit mu-1, AP mu chain family member mu1A, AP 1 complex subunit mu 1, Adaptor-related protein complex 1 subunit mu-1, Adaptor protein complex AP-1 subunit mu-1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83613-6-PBS targets AP1M1 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen AP1M1 fusion protein Ag2757 相同性解析による交差性が予測される生物種
Full Name adaptor-related protein complex 1, mu 1 subunit
Calculated molecular weight 423 aa, 49 kDa
Observed molecular weight 49 kDa
GenBank accession numberBC017469
Gene Symbol AP1M1
Gene ID (NCBI) 8907
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9BXS5
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

AP1M1, also named as CLTNM, Mu-adaptin 1 and Clathrin coat assembly protein AP47, belongs to the adaptor complexes medium subunit family. It is a subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.