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  • KD/KO Validated

AP1M1 Recombinant antibody

AP1M1 Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240553H3

Cat no : 83613-6-RR

Synonyms

AP-1 complex subunit mu-1, AP mu chain family member mu1A, AP 1 complex subunit mu 1, Adaptor-related protein complex 1 subunit mu-1, Adaptor protein complex AP-1 subunit mu-1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AP1M1 Recombinant antibody (83613-6-RR)

    WB analysis using 83613-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83613-6-RR (AP1M1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using AP1M1 Recombinant antibody (83613-6-RR)

    WB analysis of HEK-293 using 83613-6-RR

    WB result of AP1M1 antibody (83613-6-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AP1M1 transfected HEK-293 cells.

  • Western Blot (WB) analysis of various lysates using AP1M1 Recombinant antibody (83613-6-RR)

    WB analysis using 83613-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83613-6-RR (AP1M1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HeLa cells using AP1M1 Recombinant antibody (83613-6-RR)

    FC experiment of HeLa using 83613-6-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug AP1M1 Recombinant antibody (83613-6-RR, Clone:240553H3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of U-251 cells using AP1M1 Recombinant antibody (83613-6-RR)

    FC experiment of U-251 using 83613-6-RR

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug AP1M1 Recombinant antibody (83613-6-RR, Clone:240553H3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83613-6-RR

    Affinity and Kinetic Characterization of 83613-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83613-6-RR against Human AP1M1 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inHepG2 cells, HEK-293 cells, mouse testis tissue, PC-3 cells, K-562 cells, Jurkat cells, rat testis tissue
Positive FC (Intra) detected inHeLa cells, U-251 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83613-6-RR is AP1M1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen AP1M1 fusion protein Ag2757 相同性解析による交差性が予測される生物種
Full Name adaptor-related protein complex 1, mu 1 subunit
Calculated molecular weight 423 aa, 49 kDa
Observed molecular weight 49 kDa
GenBank accession numberBC017469
Gene symbol AP1M1
Gene ID (NCBI) 8907
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AP1M1, also named as CLTNM, Mu-adaptin 1 and Clathrin coat assembly protein AP47, belongs to the adaptor complexes medium subunit family. It is a subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.

Protocols

Product Specific Protocols
WB protocol for AP1M1 antibody 83613-6-RRDownload protocol
FC protocol for AP1M1 antibody 83613-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols