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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AP2A1 Polyclonal antibody (30030-1-AP)

    WB analysis using 30030-1-AP (same clone as 30030-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30030-1-AP (AP2A1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30030-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using AP2A1 Polyclonal antibody (30030-1-AP)

    WB analysis using 30030-1-AP (same clone as 30030-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30030-1-AP (AP2A1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30030-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using AP2A1 Polyclonal antibody (30030-1-AP)

    IHC staining of human stomach cancer using 30030-1-AP (same clone as 30030-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30030-1-AP (AP2A1 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30030-1-PBS in a different storage buffer formulation.

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AP2A1 Polyclonal antibody, PBS Only

AP2A1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 30030-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30030-1-PBS targets AP2A1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AP2A1 fusion protein Ag32232 相同性解析による交差性が予測される生物種
Full Name adaptor-related protein complex 2, alpha 1 subunit
Calculated molecular weight 977 aa, 108 kDa
Observed molecular weight108 kDa
GenBank accession numberBC014214
Gene Symbol AP2A1
Gene ID (NCBI) 160
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95782
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

AP2A1 encodes adaptor protein complex 2 (AP-2) subunit alpha-1, which is a 100 kDa coated vesicle protein. Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP complexes are involved in the formation of clathrin-coated vesicles (CCVs) by recruiting the scaffold protein, clathrin. AP complexes also play a pivotal role in the cargo selection by recognizing the sorting signals within the cytoplasmic tail of integral membrane proteins. AP-2 is involved in clathrin-dependent endocytosis.