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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

APBB1 Monoclonal antibody, PBS Only

APBB1 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B9G10

Cat no : 67077-1-PBS

Synonyms

APBB1, FE65, Protein Fe65, RIR

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using APBB1 Monoclonal antibody (67077-1-Ig)

    WB analysis using 67077-1-Ig (same clone as 67077-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67077-1-Ig (APBB1 antibody) at dilution of 1:1400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67077-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using APBB1 Monoclonal antibody (67077-1-Ig)

    IHC staining of mouse cerebellum using 67077-1-Ig (same clone as 67077-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67077-1-Ig (APBB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67077-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using APBB1 Monoclonal antibody (67077-1-Ig)

    IHC staining of mouse cerebellum using 67077-1-Ig (same clone as 67077-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67077-1-Ig (APBB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67077-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using APBB1 Monoclonal antibody (67077-1-Ig)

    IHC staining of mouse brain using 67077-1-Ig (same clone as 67077-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67077-1-Ig (APBB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67077-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using APBB1 Monoclonal antibody (67077-1-Ig)

    IHC staining of mouse brain using 67077-1-Ig (same clone as 67077-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67077-1-Ig (APBB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67077-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using APBB1 Monoclonal antibody (67077-1-Ig)

    IF Staining of mouse brain using 67077-1-Ig (same clone as 67077-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using APBB1 antibody (67077-1-Ig, Clone: 1B9G10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67077-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67077-1-PBS targets APBB1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen APBB1 fusion protein Ag27436 相同性解析による交差性が予測される生物種
Full Name amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65)
Calculated molecular weight 708 aa, 77 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC010854
Gene symbol APBB1
Gene ID (NCBI) 322
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

APBB1(Amyloid-beta A4 precursor protein-binding family B member 1) encoded FE65 protein. It was known as a binding partner of APP in the Alzheimer's disease studies, and expressed at high levels in brain especially in cerebellum, hippocampus, and cortex. FE65 and FE65-like (FE65L or FE65L1) proteins are cytoplasmic adaptor proteins that possess two phosphotyrosine binding domains (PTB1 and PTB2) and one WW binding domain (PMID:22429478). After phosphorylation modification, the band of FE65 protein would appear around 100 kDa. However, some tested a non-specific band at 55-60 kDa, it was refer to as FE65-like protein(PMID:12843239).