APBB1 Monoclonal antibody
APBB1 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat, Pig
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
CloneNo.
1B9G10
Cat no : 67077-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | pig brain tissue, Pig brain, Rat brain, Mouse brain |
Positive IHC detected in | mouse cerebellum tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse brain tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:3000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
67077-1-Ig targets APBB1 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.
Tested Reactivity | Human, Mouse, Rat, Pig |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | APBB1 fusion protein Ag27436 相同性解析による交差性が予測される生物種 |
Full Name | amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65) |
Calculated molecular weight | 708 aa, 77 kDa |
Observed molecular weight | 100 kDa |
GenBank accession number | BC010854 |
Gene symbol | APBB1 |
Gene ID (NCBI) | 322 |
RRID | AB_2882385 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
APBB1(Amyloid-beta A4 precursor protein-binding family B member 1) encoded FE65 protein. It was known as a binding partner of APP in the Alzheimer's disease studies, and expressed at high levels in brain especially in cerebellum, hippocampus, and cortex. FE65 and FE65-like (FE65L or FE65L1) proteins are cytoplasmic adaptor proteins that possess two phosphotyrosine binding domains (PTB1 and PTB2) and one WW binding domain (PMID:22429478). After phosphorylation modification, the band of FE65 protein would appear around 100 kDa. However, some tested a non-specific band at 55-60 kDa, it was refer to as FE65-like protein(PMID:12843239).
Protocols
Product Specific Protocols | |
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WB protocol for APBB1 antibody 67077-1-Ig | Download protocol |
IHC protocol for APBB1 antibody 67077-1-Ig | Download protocol |
IF protocol for APBB1 antibody 67077-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |