CoraLite® Plus 488-conjugated APBB1 Monoclonal antibody
APBB1 Monoclonal Antibody for IF-P
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat, Pig
Applications
IF-P
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1B9G10
Cat no : CL488-67077
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-67077 targets APBB1 in IF-P applications and shows reactivity with Human, Mouse, Rat, Pig samples.
| Tested Reactivity | Human, Mouse, Rat, Pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | APBB1 fusion protein Ag27436 相同性解析による交差性が予測される生物種 |
| Full Name | amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65) |
| Calculated molecular weight | 708 aa, 77 kDa |
| GenBank accession number | BC010854 |
| Gene symbol | APBB1 |
| Gene ID (NCBI) | 322 |
| RRID | AB_2919418 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
APBB1(Amyloid-beta A4 precursor protein-binding family B member 1) encoded FE65 protein. It was known as a binding partner of APP in the Alzheimer's disease studies, and expressed at high levels in brain especially in cerebellum, hippocampus, and cortex. FE65 and FE65-like (FE65L or FE65L1) proteins are cytoplasmic adaptor proteins that possess two phosphotyrosine binding domains (PTB1 and PTB2) and one WW binding domain (PMID:22429478). After phosphorylation modification, the band of FE65 protein would appear around 100 kDa. However, some tested a non-specific band at 55-60 kDa, it was refer to as FE65-like protein(PMID:12843239).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 APBB1 antibody CL488-67077 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
