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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Serum amyloid P component Monoclonal antibody

Serum amyloid P component Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1A1D10

Cat no : 66084-1-Ig

Synonyms

APCS, SAP, PTX2, amyloid P component, serum, 9.5S alpha-1-glycoprotein

Validation Data Gallery

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  • Western Blot (WB) analysis of human kidney tissue using Serum amyloid P component Monoclonal antibody (66084-1-Ig)

    WB analysis of human kidney using 66084-1-Ig

    human kidney tissue were subjected to SDS PAGE followed by western blot with 66084-1-Ig (Serum amyloid P component antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human plasma using Serum amyloid P component Monoclonal antibody (66084-1-Ig)

    WB analysis of human plasma using 66084-1-Ig

    human blood were subjected to SDS PAGE followed by western blot with 66084-1-Ig (Serum amyloid P component antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Serum amyloid P component Monoclonal antibody (66084-1-Ig)

    IHC staining of human kidney using 66084-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney using 66084-1-Ig(Serum amyloid P component antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using Serum amyloid P component Monoclonal antibody (66084-1-Ig)

    IHC staining of human kidney using 66084-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney using 66084-1-Ig(Serum amyloid P component antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using Serum amyloid P component Monoclonal antibody (66084-1-Ig)

    IHC staining of human liver using 66084-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66084-1-Ig (Serum amyloid P component antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using Serum amyloid P component Monoclonal antibody (66084-1-Ig)

    IHC staining of human liver using 66084-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66084-1-Ig (Serum amyloid P component antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using Serum amyloid P component Monoclonal antibody (66084-1-Ig)

    IF Staining of human liver cancer using 66084-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Serum amyloid P component antibody (66084-1-Ig, Clone: 1A1D10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using Serum amyloid P component Monoclonal antibody (66084-1-Ig)

    IF Staining of human liver cancer using 66084-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Serum amyloid P component antibody (66084-1-Ig, Clone: 1A1D10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using Serum amyloid P component Monoclonal antibody (66084-1-Ig)

    IF Staining of mouse spleen using 66084-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using Serum amyloid P component antibody (66084-1-Ig, Clone: 1A1D10 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human liver tissue using Serum amyloid P component Monoclonal antibody (66084-1-Ig)

    IF Staining of human liver using 66084-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using Serum amyloid P component antibody (66084-1-Ig, Clone: 1A1D10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inhuman kidney tissue, human plasma
Positive IHC detected inhuman liver tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver cancer tissue, human liver tissue, mouse spleen tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

66084-1-Ig targets Serum amyloid P component in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Serum amyloid P component fusion protein Ag14957 相同性解析による交差性が予測される生物種
Full Name amyloid P component, serum
Calculated molecular weight 223 aa, 25 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC007058
Gene symbol Serum amyloid P component
Gene ID (NCBI) 325
RRIDAB_11182271
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Serum amyloid P component (SAP), a member of pentraxin family, is a constitutive serum protein that is synthesized by hepatocytes. It is an acute phase protein, structurally related to C-reactive protein. SAP is a calcium-dependent ligand binding protein, which can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.

Protocols

Product Specific Protocols
WB protocol for Serum amyloid P component antibody 66084-1-IgDownload protocol
IHC protocol for Serum amyloid P component antibody 66084-1-IgDownload protocol
IF protocol for Serum amyloid P component antibody 66084-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Clin Chim Acta

Combined analysis of serum SAP and PRSS2 for the differential diagnosis of CD and UC

Authors - Weifeng Wang
View Article