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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using CoraLite®594-conjugated Serum amyloid P component (CL594-66084)

    IF Staining of mouse spleen using CL594-66084

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CoraLite®594 Serum amyloid P component antibody (CL594-66084, Clone: 1A1D10 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using CoraLite®594-conjugated Serum amyloid P component (CL594-66084)

    IF Staining of human liver cancer using CL594-66084

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®594 Serum amyloid P component antibody (CL594-66084, Clone: 1A1D10 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using CoraLite®594-conjugated Serum amyloid P component (CL594-66084)

    IF Staining of human liver cancer using CL594-66084

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®594 Serum amyloid P component antibody (CL594-66084, Clone: 1A1D10 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of human liver tissue using CoraLite®594-conjugated Serum amyloid P component (CL594-66084)

    IF Staining of human liver using CL594-66084

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using CoraLite®594 Serum amyloid P component antibody (CL594-66084, Clone: 1A1D10 ) at dilution of 1:200.

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CoraLite®594-conjugated Serum amyloid P component Monoclonal antibody

Serum amyloid P component Monoclonal Antibody for IF-P
Cat No. CL594-66084
Clone No.1A1D10

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

IF-P

Synonyms

APCS, SAP, PTX2, amyloid P component, serum, 9.5S alpha-1-glycoprotein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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Tested Applications

Positive IF-P detected inhuman liver cancer tissue, human liver tissue, mouse spleen tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66084 targets Serum amyloid P component in IF-P applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Serum amyloid P component fusion protein Ag14957 相同性解析による交差性が予測される生物種
Full Name amyloid P component, serum
Calculated molecular weight 223 aa, 25 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC007058
Gene Symbol Serum amyloid P component
Gene ID (NCBI) 325
RRIDAB_2934715
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP02743
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Serum amyloid P component (SAP), a member of pentraxin family, is a constitutive serum protein that is synthesized by hepatocytes. It is an acute phase protein, structurally related to C-reactive protein. SAP is a calcium-dependent ligand binding protein, which can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.

Protocols

Product Specific Protocols
IF protocol for CL594 Serum amyloid P component antibody CL594-66084Download protocol
Standard Protocols
Click here to view our Standard Protocols