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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using APH1A Polyclonal antibody (11643-1-AP)

    WB analysis using 11643-1-AP (same clone as 11643-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11643-1-AP (APH1A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11643-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using APH1A Polyclonal antibody (11643-1-AP)

    WB analysis using 11643-1-AP (same clone as 11643-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11643-1-AP (APH1A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11643-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using APH1A Polyclonal antibody (11643-1-AP)

    WB analysis of HeLa using 11643-1-AP (same clone as 11643-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 11643-1-AP (APH1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11643-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using APH1A Polyclonal antibody (11643-1-AP)

    WB analysis of human brain using 11643-1-AP (same clone as 11643-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 11643-1-AP (APH1A antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11643-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using APH1A Polyclonal antibody (11643-1-AP)

    IP experiment of HeLa using 11643-1-AP (same clone as 11643-1-PBS)

    IP result of anti-APH1A (IP:11643-1-AP, 4ug; Detection:11643-1-AP 1:4000) with HeLa cells lysate 1600 ug. This data was developed using the same antibody clone with 11643-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using APH1A Polyclonal antibody (11643-1-AP)

    IHC staining of human stomach using 11643-1-AP (same clone as 11643-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11643-1-AP (APH1A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11643-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using APH1A Polyclonal antibody (11643-1-AP)

    IHC staining of human stomach using 11643-1-AP (same clone as 11643-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11643-1-AP (APH1A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11643-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using APH1A Polyclonal antibody (11643-1-AP)

    IF Staining of HeLa using 11643-1-AP (same clone as 11643-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using APH1A antibody (11643-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11643-1-PBS in a different storage buffer formulation.

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APH1A Polyclonal antibody, PBS Only

APH1A Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 11643-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

CGI-78, CGI 78, Aph-1alpha, APH-1a, Aph 1alpha

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11643-1-PBS targets APH1A in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen APH1A fusion protein Ag2238 相同性解析による交差性が予測される生物種
Full Name anterior pharynx defective 1 homolog A (C. elegans)
Calculated molecular weight 265 aa, 29 kDa
Observed molecular weight27-32 kDa
GenBank accession numberBC015568
Gene Symbol APH1A
Gene ID (NCBI) 51107
RRIDAB_2242861
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96BI3
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.