APOA4 Polyclonal antibody
APOA4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 17996-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human plasma tissue, HepG2 cells, human plasma |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 6 publications below |
| IHC | See 2 publications below |
Product Information
17996-1-AP targets APOA4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | APOA4 fusion protein Ag12454 相同性解析による交差性が予測される生物種 |
| Full Name | apolipoprotein A-IV |
| Calculated molecular weight | 396 aa, 45 kDa |
| Observed molecular weight | 45-46 kDa |
| GenBank accession number | BC074764 |
| Gene symbol | APOA4 |
| Gene ID (NCBI) | 337 |
| RRID | AB_10596638 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
APOA4 is a major protein component of chylomicrons in postprandial lymph and plasma. It is synthesized in the small intestine and the liver, attached to chylomicrons by enterocytes, and secreted into intestinal lymph during fat absorption. APOA4 is intimately involved in metabolism and has protective roles in cardiovascular diseases like atherosclerosis. APOA4 can promote ins secretion and maintain glucose homeostasis. It also has potent antioxidant and anti-inflammatory properties.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for APOA4 antibody 17996-1-AP | Download protocol |
| IHC protocol for APOA4 antibody 17996-1-AP | Download protocol |
| IF protocol for APOA4 antibody 17996-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Exp Med Single-cell transcriptome analysis reveals differential nutrient absorption functions in human intestine. | ||
JCI Insight The distinct metabolism between large and small HDL indicates unique origins of human apolipoprotein A4 | ||
Int J Mol Sci Serum APOA4 Pharmacodynamically Represents Administered Recombinant Human Hepatocyte Growth Factor (E3112). | ||
Alcohol Hepatic COX-2 expression protects mice from an alcohol-high fat diet-induced metabolic disorder by involving protein acetylation related energy metabolism. | ||
Cardiol Young Differential protein analysis of serum exosomes post-intravenous immunoglobulin therapy in patients with Kawasaki disease. |
