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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FITC Plus-conjugated APOA4 Polyclonal antibody

APOA4 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

FITC Plus Fluorescent Dye

Cat no : FITC-17996

Synonyms

APOA4, apolipoprotein A IV

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FITC Plus-conjugated APOA4 Polyclonal antibody (FITC-17996)

    IF Staining of HepG2 using FITC-17996

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FITC Plus APOA4 antibody (FITC-17996) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

FITC-17996 targets APOA4 in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen APOA4 fusion protein Ag12454 相同性解析による交差性が予測される生物種
Full Name apolipoprotein A-IV
Calculated molecular weight 396 aa, 45 kDa
Observed molecular weight 45-46 kDa
GenBank accession numberBC074764
Gene symbol APOA4
Gene ID (NCBI) 337
Conjugate FITC Plus Fluorescent Dye
Excitation/Emission maxima wavelengths495 nm / 524 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

APOA4 is a major protein component of chylomicrons in postprandial lymph and plasma. It is synthesized in the small intestine and the liver, attached to chylomicrons by enterocytes, and secreted into intestinal lymph during fat absorption. APOA4 is intimately involved in metabolism and has protective roles in cardiovascular diseases like atherosclerosis. APOA4 can promote ins secretion and maintain glucose homeostasis. It also has potent antioxidant and anti-inflammatory properties.

Protocols

Product Specific Protocols
IF protocol for FITC Plus APOA4 antibody FITC-17996Download protocol
Standard Protocols
Click here to view our Standard Protocols