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Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using APOA4 Monoclonal antibody (66252-1-Ig)

    WB analysis of human plasma using 66252-1-Ig (same clone as 66252-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 66252-1-Ig (APOA4 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66252-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L02 cells using APOA4 Monoclonal antibody (66252-1-Ig)

    WB analysis of L02 using 66252-1-Ig (same clone as 66252-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 66252-1-Ig (APOA4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66252-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using APOA4 Monoclonal antibody (66252-1-Ig)

    IHC staining of mouse small intestine using 66252-1-Ig (same clone as 66252-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 66252-1-Ig (APOA4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66252-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using APOA4 Monoclonal antibody (66252-1-Ig)

    IHC staining of mouse small intestine using 66252-1-Ig (same clone as 66252-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 66252-1-Ig (APOA4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66252-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using APOA4 Monoclonal antibody (66252-1-Ig)

    IF Staining of SH-SY5Y using 66252-1-Ig (same clone as 66252-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using APOA4 antibody (66252-1-Ig, Clone: 3A9A8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66252-1-PBS in a different storage buffer formulation.

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APOA4 Monoclonal antibody, PBS Only

APOA4 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66252-1-PBS
Clone No.3A9A8

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

APOA4, apolipoprotein A IV

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66252-1-PBS targets APOA4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen APOA4 fusion protein Ag12454 相同性解析による交差性が予測される生物種
Full Name apolipoprotein A-IV
Calculated molecular weight 396 aa, 45 kDa
Observed molecular weight 45-46 kDa
GenBank accession numberBC074764
Gene Symbol APOA4
Gene ID (NCBI) 337
RRIDAB_2881640
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP06727
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

APOA4 is a major protein component of chylomicrons in postprandial lymph and plasma. It is synthesized in the small intestine and the liver, attached to chylomicrons by enterocytes, and secreted into intestinal lymph during fat absorption. APOA4 is intimately involved in metabolism and has protective roles in cardiovascular diseases like atherosclerosis. APOA4 can promote INS secretion and maintain glucose homeostasis. It also has potent antioxidant and anti-inflammatory properties.