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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

APOA4 Monoclonal antibody

APOA4 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3A9A8

Cat no : 66252-1-Ig

Synonyms

APOA4, apolipoprotein A IV

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using APOA4 Monoclonal antibody (66252-1-Ig)

    WB analysis of human plasma using 66252-1-Ig

    human plasma were subjected to SDS PAGE followed by western blot with 66252-1-Ig (APOA4 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using APOA4 Monoclonal antibody (66252-1-Ig)

    WB analysis of L02 using 66252-1-Ig

    L02 cells were subjected to SDS PAGE followed by western blot with 66252-1-Ig (APOA4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using APOA4 Monoclonal antibody (66252-1-Ig)

    IHC staining of mouse small intestine using 66252-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 66252-1-Ig (APOA4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using APOA4 Monoclonal antibody (66252-1-Ig)

    IHC staining of mouse small intestine using 66252-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 66252-1-Ig (APOA4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using APOA4 Monoclonal antibody (66252-1-Ig)

    IF Staining of SH-SY5Y using 66252-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using APOA4 antibody (66252-1-Ig, Clone: 3A9A8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Tested Applications

Positive WB detected inhuman plasma tissue, L02 cells
Positive IHC detected inmouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66252-1-Ig targets APOA4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen APOA4 fusion protein Ag12454 相同性解析による交差性が予測される生物種
Full Name apolipoprotein A-IV
Calculated molecular weight 396 aa, 45 kDa
Observed molecular weight 45-46 kDa
GenBank accession numberBC074764
Gene symbol APOA4
Gene ID (NCBI) 337
RRIDAB_2881640
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

APOA4 is a major protein component of chylomicrons in postprandial lymph and plasma. It is synthesized in the small intestine and the liver, attached to chylomicrons by enterocytes, and secreted into intestinal lymph during fat absorption. APOA4 is intimately involved in metabolism and has protective roles in cardiovascular diseases like atherosclerosis. APOA4 can promote INS secretion and maintain glucose homeostasis. It also has potent antioxidant and anti-inflammatory properties.

Protocols

Product Specific Protocols
WB protocol for APOA4 antibody 66252-1-IgDownload protocol
IHC protocol for APOA4 antibody 66252-1-IgDownload protocol
IF protocol for APOA4 antibody 66252-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols