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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using Biotin-conjugated APOA4 Monoclonal antibody (Biotin-66252)

    IHC staining of mouse small intestine using Biotin-66252

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using Biotin-66252 (APOA4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

Biotin-conjugated APOA4 Monoclonal antibody

APOA4 Monoclonal Antibody for IHC
Cat No. Biotin-66252
Clone No.3A9A8

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IHC

Synonyms

APOA4, apolipoprotein A IV

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Biotin
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inmouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-66252 targets APOA4 in IHC applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen APOA4 fusion protein Ag12454 相同性解析による交差性が予測される生物種
Full Name apolipoprotein A-IV
Calculated molecular weight 396 aa, 45 kDa
Observed molecular weight 45-46 kDa
GenBank accession numberBC074764
Gene Symbol APOA4
Gene ID (NCBI) 337
RRIDAB_3672377
Conjugate Biotin
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP06727
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

APOA4 is a major protein component of chylomicrons in postprandial lymph and plasma. It is synthesized in the small intestine and the liver, attached to chylomicrons by enterocytes, and secreted into intestinal lymph during fat absorption. APOA4 is intimately involved in metabolism and has protective roles in cardiovascular diseases like atherosclerosis. APOA4 can promote INS secretion and maintain glucose homeostasis. It also has potent antioxidant and anti-inflammatory properties.

Protocols

Product Specific Protocols
IHC protocol for Biotin APOA4 antibody Biotin-66252Download protocol
Standard Protocols
Click here to view our Standard Protocols