Biotin-conjugated APOA4 Monoclonal antibody
APOA4 Monoclonal Antibody for IHC
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse
Applications
IHC
Conjugate
Biotin
CloneNo.
3A9A8
Cat no : Biotin-66252
Synonyms
Validation Data Gallery
Tested Applications
| Positive IHC detected in | mouse small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
Biotin-66252 targets APOA4 in IHC applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | APOA4 fusion protein Ag12454 相同性解析による交差性が予測される生物種 |
| Full Name | apolipoprotein A-IV |
| Calculated molecular weight | 396 aa, 45 kDa |
| Observed molecular weight | 45-46 kDa |
| GenBank accession number | BC074764 |
| Gene symbol | APOA4 |
| Gene ID (NCBI) | 337 |
| Conjugate | Biotin |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
APOA4 is a major protein component of chylomicrons in postprandial lymph and plasma. It is synthesized in the small intestine and the liver, attached to chylomicrons by enterocytes, and secreted into intestinal lymph during fat absorption. APOA4 is intimately involved in metabolism and has protective roles in cardiovascular diseases like atherosclerosis. APOA4 can promote INS secretion and maintain glucose homeostasis. It also has potent antioxidant and anti-inflammatory properties.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Biotin APOA4 antibody Biotin-66252 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
