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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

APOB Recombinant antibody

APOB Recombinant Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

240884H4

Cat no : 84047-1-RR

Synonyms

Apolipoprotein B, ApoB48, ApoB100, Apo B-100, Apo B

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using APOB Recombinant antibody (84047-1-RR)

    WB analysis of mouse liver using 84047-1-RR

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 84047-1-RR (APOB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using APOB Recombinant antibody (84047-1-RR)

    IHC staining of mouse liver using 84047-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84047-1-RR (APOB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using APOB Recombinant antibody (84047-1-RR)

    IF Staining of mouse liver using 84047-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using APOB antibody (84047-1-RR, Clone: 240884H4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using APOB Recombinant antibody (84047-1-RR)

    IF Staining of mouse liver using 84047-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using APOB antibody (84047-1-RR, Clone: 240884H4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse liver tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84047-1-RR targets APOB in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name apolipoprotein B (including Ag(x) antigen)
Calculated molecular weight 516 kDa
Observed molecular weight150-250 kDa, 400-520 kDa
GenBank accession numberNM_000384
Gene symbol APOB
Gene ID (NCBI) 338
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The apolipoprotein B (APOB) is a plasma protein synthesized primarily in the liver and intestine and play an important role in lipid and cholesterol metabolism. The APOB encodes two different isoproteins through mRNA editing, APOB-48 and APOB-100. APOB-48 and APOB-100 is present in both human liver and intestine. APOB-100 is essential for the assembly of VLDL in the liver. APOB-48 is essential for the assembly of chylomicrons in the intestine. It is well established that APOB-100 levels are associated with coronary heart disease. This antibody recognizes both of APOB-48, APOB-100 (PMID:11839763, PMID:2450346).

Protocols

Product Specific Protocols
WB protocol for APOB antibody 84047-1-RRDownload protocol
IHC protocol for APOB antibody 84047-1-RRDownload protocol
IF protocol for APOB antibody 84047-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols