• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated APOB Recombinant antibody

APOB Recombinant Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

240884H4

Cat no : CL488-84047

Synonyms

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using CoraLite® Plus 488-conjugated APOB Recombinant ant (CL488-84047)

    IF Staining of mouse liver using CL488-84047

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using CoraLite® Plus 488 APOB antibody (CL488-84047, Clone: 240884H4 ) at dilution of 1:100. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using CoraLite® Plus 488-conjugated APOB Recombinant ant (CL488-84047)

    IF Staining of mouse liver using CL488-84047

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using CoraLite® Plus 488 APOB antibody (CL488-84047, Clone: 240884H4 ) at dilution of 1:100. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

☆大好評トライアルサイズ ¥20,000～！ >>> 一次抗体特別価格キャンペーン開催中！

Tested Applications

Positive IF-P detected inmouse liver tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-84047 targets APOB in IF-P applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name apolipoprotein B (including Ag(x) antigen)
Calculated molecular weight 516 kDa
Observed molecular weight150-250 kDa, 400-520 kDa
GenBank accession numberNM_000384
Gene symbol APOB
Gene ID (NCBI) 338
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The apolipoprotein B (APOB) is a plasma protein synthesized primarily in the liver and intestine and play an important role in lipid and cholesterol metabolism. The APOB encodes two different isoproteins through mRNA editing, APOB-48 and APOB-100. APOB-48 and APOB-100 is present in both human liver and intestine. APOB-100 is essential for the assembly of VLDL in the liver. APOB-48 is essential for the assembly of chylomicrons in the intestine. It is well established that APOB-100 levels are associated with coronary heart disease. This antibody recognizes both of APOB-48, APOB-100 (PMID:11839763, PMID:2450346).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 APOB antibody CL488-84047Download protocol
Standard Protocols
Click here to view our Standard Protocols