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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HRP-conjugated APOB Polyclonal antibody

APOB Polyclonal Antibody for WB

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB

Conjugate

HRP

Cat no : HRP-20578

Synonyms

Apo B 100, Apo B 48, APOB, ApoB100, Apolipoprotein B 100, FLDB

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using HRP-conjugated APOB Polyclonal antibody (HRP-20578)

    WB analysis of human plasma using HRP-20578

    human plasma were subjected to SDS PAGE followed by western blot with HRP-20578 (APOB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using HRP-conjugated APOB Polyclonal antibody (HRP-20578)

    WB analysis of mouse liver using HRP-20578

    mouse liver tissue were subjected to SDS PAGE followed by western blot with HRP-20578 (APOB antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using HRP-conjugated APOB Polyclonal antibody (HRP-20578)

    WB analysis of mouse liver using HRP-20578

    mouse liver tissue were subjected to SDS PAGE followed by western blot with HRP-20578 (APOB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inmouse liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

HRP-20578 targets APOB in WB applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name apolipoprotein B (including Ag(x) antigen)
Calculated molecular weight 516 kDa
Observed molecular weight150-250 kDa, 400-520 kDa
GenBank accession numberNM_000384
Gene symbol APOB
Gene ID (NCBI) 338
Conjugate HRP
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The apolipoprotein B (APOB) is a plasma protein synthesized primarily in the liver and intestine and play an important role in lipid and cholesterol metabolism. The APOB encodes two different isoproteins through mRNA editing, APOB-48 and APOB-100. APOB-48 and APOB-100 is present in both human liver and intestine. APOB-100 is essential for the assembly of VLDL in the liver. APOB-48 is essential for the assembly of chylomicrons in the intestine. It is well established that APOB-100 levels are associated with coronary heart disease. This antibody recognizes both of APOB-48, APOB-100 and APOB-27.6 (PMID:11839763, PMID:2450346).

Protocols

Product Specific Protocols
WB protocol for HRP APOB antibody HRP-20578Download protocol
Standard Protocols
Click here to view our Standard Protocols