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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

APOE Recombinant antibody, PBS Only

APOE Recombinant Antibody for IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

242310B2

Cat no : 84775-7-PBS

Synonyms

ApolipoproteinE, apolipoprotein E, apoe4, Apo-E, Apo E

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse liver tissue using APOE Recombinant antibody (84775-7-RR)

    IHC staining of mouse liver using 84775-7-RR (same clone as 84775-7-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84775-7-RR (APOE antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84775-7-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using APOE Recombinant antibody (84775-7-RR)

    IHC staining of mouse liver using 84775-7-RR (same clone as 84775-7-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84775-7-RR (APOE antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84775-7-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using APOE Recombinant antibody (84775-7-RR)

    IF Staining of HepG2 using 84775-7-RR (same clone as 84775-7-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using APOE antibody (84775-7-RR, Clone: 242310B2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84775-7-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84775-7-RR

    Affinity and Kinetic Characterization of 84775-7-RR

    Biolayer interferometry (BLl) kinetic assays of 84775-7-RR against Human APOE were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84775-7-PBS targets APOE in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name Apolipoprotein E
Calculated molecular weight 36 kDa
GenBank accession numberBC003557
Gene symbol APOE
Gene ID (NCBI) 348
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The apolipoprotein E (APOE) is a 299-amino acid polypeptide that mediates the binding, internalization, and catabolism of lipoprotein particles, and also serves as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues. The very strong association of the APOE ɛ4 allele with AD risk and its role in the accumulation of amyloid β in brains of people and animal models solidify the biological relevance of APOE isoforms but do not provide mechanistic insight (PMID: 9831633).