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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse liver tissue using APOE Recombinant antibody (84775-7-RR)

    IHC staining of mouse liver using 84775-7-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84775-7-RR (APOE antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using APOE Recombinant antibody (84775-7-RR)

    IHC staining of mouse liver using 84775-7-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84775-7-RR (APOE antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using APOE Recombinant antibody (84775-7-RR)

    IF Staining of HepG2 using 84775-7-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using APOE antibody (84775-7-RR, Clone: 242310B2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84775-7-RR

    Affinity and Kinetic Characterization of 84775-7-RR

    Biolayer interferometry (BLl) kinetic assays of 84775-7-RR against Human APOE were performed. The affinity constant is below 1 pM.

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APOE Recombinant antibody

APOE Recombinant Antibody for IHC, IF/ICC, ELISA
Cat No. 84775-7-RR
Clone No.242310B2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Synonyms

ApolipoproteinE, apolipoprotein E, apoe4, Apo-E, Apo E

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 647
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84775-7-RR targets APOE in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name Apolipoprotein E
Calculated molecular weight 36 kDa
GenBank accession numberBC003557
Gene Symbol APOE
Gene ID (NCBI) 348
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP02649
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The apolipoprotein E (APOE) is a 299-amino acid polypeptide that mediates the binding, internalization, and catabolism of lipoprotein particles, and also serves as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues. The very strong association of the APOE ɛ4 allele with AD risk and its role in the accumulation of amyloid β in brains of people and animal models solidify the biological relevance of APOE isoforms but do not provide mechanistic insight (PMID: 9831633).

Protocols

Product Specific Protocols
IHC protocol for APOE antibody 84775-7-RRDownload protocol
IF protocol for APOE antibody 84775-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols