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  • Featured Product
  • KD/KO Validated

CoraLite®555-conjugated APOE Monoclonal antibody

APOE Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

1B2C9

Cat no : CL555-66830

Synonyms

ApolipoproteinE, apolipoprotein E, apoe4, Apo-E, Apo E

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of Caco-2 cells using CoraLite®555-conjugated APOE Monoclonal antibody (CL555-66830)

    IF Staining of Caco-2 using CL555-66830

    Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using CoraLite®555 APOE antibody (CL555-66830, Clone: 1B2C9 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite®555-conjugated APOE Monoclonal antibody (CL555-66830)

    FC experiment of HepG2 using CL555-66830

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®555-conjugated APOE Monoclonal antibody (CL555-66830, Clone:1B2C9)(red), or 0.4 ug CoraLite®555 Mouse IgG1 Isotype Control (MOPC-21) (CL555-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inCaco-2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-66830 targets APOE in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen APOE fusion protein Ag28186 相同性解析による交差性が予測される生物種
Full Name Apolipoprotein E
Calculated molecular weight 36 kDa
Observed molecular weight 34-36 kDa
GenBank accession numberBC003557
Gene symbol APOE
Gene ID (NCBI) 348
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The apolipoprotein E (APOE) is a 299-amino acid polypeptide that mediates the binding, internalization, and catabolism of lipoprotein particles, and also serve as a ligand for the LDL (APO B/E) receptor and for the specific APOE receptor (chylomicron remnant) of hepatic tissues. The very strong association of the APOE ɛ4 allele with AD risk and its role in the accumulation of amyloid β in brains of people and animal models solidify the biological relevance of APOE isoforms but do not provide mechanistic insight.

Protocols

Product Specific Protocols
IF protocol for CL555 APOE antibody CL555-66830Download protocol
Standard Protocols
Click here to view our Standard Protocols