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  • Featured Product
  • KD/KO Validated

APPL1 Polyclonal antibody

APPL1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 19885-1-AP

Synonyms

DIP13alpha, DIP13A, Dip13 alpha, DCC-interacting protein 13-alpha, APPL

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using APPL1 Polyclonal antibody (19885-1-AP)

    WB analysis of human brain using 19885-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 19885-1-AP (APPL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using APPL1 Polyclonal antibody (19885-1-AP)

    WB analysis of HEK-293 using 19885-1-AP

    WB result of APPL1 antibody (19885-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-APPL1 transfected HEK-293 cells.

  • Western Blot (WB) analysis of HT-1080 cells using APPL1 Polyclonal antibody (19885-1-AP)

    WB analysis of HT-1080 using 19885-1-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 19885-1-AP (APPL1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using APPL1 Polyclonal antibody (19885-1-AP)

    WB analysis of HeLa using 19885-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 19885-1-AP (APPL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using APPL1 Polyclonal antibody (19885-1-AP)

    WB analysis of human heart using 19885-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 19885-1-AP (APPL1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using APPL1 Polyclonal antibody (19885-1-AP)

    WB analysis of HEK-293 using 19885-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 19885-1-AP (APPL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using APPL1 Polyclonal antibody (19885-1-AP)

    WB analysis of mouse brain using 19885-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 19885-1-AP (APPL1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using APPL1 Polyclonal antibody (19885-1-AP)

    IP experiment of mouse brain using 19885-1-AP

    IP result of anti-APPL1 (IP:19885-1-AP, 4ug; Detection:19885-1-AP 1:1000) with mouse brain tissue lysate 2640ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using APPL1 Polyclonal antibody (19885-1-AP)

    IHC staining of human breast cancer using 19885-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 19885-1-AP (APPL1 Antibody) at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using APPL1 Polyclonal antibody (19885-1-AP)

    IF Staining of HepG2 using 19885-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using APPL1 antibody (19885-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using APPL1 Polyclonal antibody (19885-1-AP)

    IF Staining of HepG2 using 19885-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using APPL1 antibody (19885-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Flow cytometry (FC) experiment of HepG2 cells using APPL1 Polyclonal antibody (19885-1-AP)

    FC experiment of HepG2 using 19885-1-AP

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human APPL1 (19885-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive WB detected inhuman brain tissue, HEK-293 cells, HeLa cells, human heart tissue, HT-1080 cells, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19885-1-AP targets APPL1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen APPL1 fusion protein Ag13703 相同性解析による交差性が予測される生物種
Full Name adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1
Calculated molecular weight 709 aa, 80 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC028599
Gene symbol APPL1
Gene ID (NCBI) 26060
RRIDAB_10637848
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1), a binding partner of Akt2 and an important regulator of ins signaling, plays a key role in the regulation of ins secretion [PMID:22615370]. APPL1 interacts with adiponectin receptors and mediates the ins-sensitizing effects of adiponectin in muscle and endothelial cells. It also participates in nuclear signaling and transcriptional regulation, mostly by modulating the activity of various nuclear factors [PMID:22685329]. Apart from its role in endocytosis and endosomal transport, APPL1 was reported to undergo nucleocytoplasmic shuttling and participate in transcriptional regulation, e.g. by interactions with histone deacetylases (HDACs) [PMID:19686092].

Protocols

Product Specific Protocols
WB protocol for APPL1 antibody 19885-1-APDownload protocol
IHC protocol for APPL1 antibody 19885-1-APDownload protocol
IF protocol for APPL1 antibody 19885-1-APDownload protocol
IP protocol for APPL1 antibody 19885-1-APDownload protocol
FC protocol for APPL1 antibody 19885-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols