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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated APPL1 Polyclonal antibody

APPL1 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-19885

Synonyms

APPL, APPL1, Dip13 alpha, DIP13A, DIP13alpha, KIAA1428

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated APPL1 Polyclonal ant (CL488-19885)

    IF Staining of HepG2 using CL488-19885

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 APPL1 antibody (CL488-19885) at dilution of 1:200, CL594-phalloidin (red).

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Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-19885 targets APPL1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen APPL1 fusion protein Ag13703 相同性解析による交差性が予測される生物種
Full Name adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1
Calculated molecular weight 709 aa, 80 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC028599
Gene symbol APPL1
Gene ID (NCBI) 26060
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1), a binding partner of Akt2 and an important regulator of ins signaling, plays a key role in the regulation of ins secretion [PMID:22615370]. APPL1 interacts with adiponectin receptors and mediates the ins-sensitizing effects of adiponectin in muscle and endothelial cells. It also participates in nuclear signaling and transcriptional regulation, mostly by modulating the activity of various nuclear factors [PMID:22685329]. Apart from its role in endocytosis and endosomal transport, APPL1 was reported to undergo nucleocytoplasmic shuttling and participate in transcriptional regulation, e.g. by interactions with histone deacetylases (HDACs) [PMID:19686092].

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 APPL1 antibody CL488-19885Download protocol
Standard Protocols
Click here to view our Standard Protocols