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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated APPL1 Monoclonal ant (CL488-68195)

    IF Staining of HepG2 using CL488-68195

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 APPL1 antibody (CL488-68195, Clone: 1B7B11 ) at dilution of 1:50.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

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  • KD/KO Validated

CoraLite® Plus 488-conjugated APPL1 Monoclonal antibody

APPL1 Monoclonal Antibody for IF/ICC
Cat No. CL488-68195
Clone No.1B7B11

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, rabbit, pig

Applications

IF/ICC

Synonyms

APPL, APPL1, Dip13 alpha, DIP13A, DIP13alpha, KIAA1428

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68195 targets APPL1 in IF/ICC applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

Tested Reactivity Human, mouse, rat, rabbit, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen APPL1 fusion protein Ag3334 相同性解析による交差性が予測される生物種
Full Name adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1
Calculated molecular weight 709 aa, 80 kDa
Observed molecular weight80 kDa
GenBank accession numberBC028599
Gene Symbol APPL1
Gene ID (NCBI) 26060
RRIDAB_3084437
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UKG1
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1), a binding partner of Akt2 and an important regulator of INS signaling, plays a key role in the regulation of INS secretion [PMID:22615370]. APPL1 interacts with adiponectin receptors and mediates the INS-sensitizing effects of adiponectin in muscle and endothelial cells. It also participates in nuclear signaling and transcriptional regulation, mostly by modulating the activity of various nuclear factors [PMID:22685329]. Apart from its role in endocytosis and endosomal transport, APPL1 was reported to undergo nucleocytoplasmic shuttling and participate in transcriptional regulation, e.g. by interactions with histone deacetylases (HDACs) [PMID:19686092].

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 APPL1 antibody CL488-68195Download protocol
Standard Protocols
Click here to view our Standard Protocols