- Featured Product
- KD/KO Validated
CoraLite® Plus 488-conjugated APPL1 Monoclonal antibody
APPL1 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG2b
Reactivity
Human, mouse, rat, rabbit, pig
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1B7B11
Cat no : CL488-68195
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-68195 targets APPL1 in IF/ICC applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.
| Tested Reactivity | Human, mouse, rat, rabbit, pig |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | APPL1 fusion protein Ag3334 相同性解析による交差性が予測される生物種 |
| Full Name | adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 |
| Calculated molecular weight | 709 aa, 80 kDa |
| Observed molecular weight | 80 kDa |
| GenBank accession number | BC028599 |
| Gene symbol | APPL1 |
| Gene ID (NCBI) | 26060 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1), a binding partner of Akt2 and an important regulator of INS signaling, plays a key role in the regulation of INS secretion [PMID:22615370]. APPL1 interacts with adiponectin receptors and mediates the INS-sensitizing effects of adiponectin in muscle and endothelial cells. It also participates in nuclear signaling and transcriptional regulation, mostly by modulating the activity of various nuclear factors [PMID:22685329]. Apart from its role in endocytosis and endosomal transport, APPL1 was reported to undergo nucleocytoplasmic shuttling and participate in transcriptional regulation, e.g. by interactions with histone deacetylases (HDACs) [PMID:19686092].
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 APPL1 antibody CL488-68195 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
