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Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using AQP1 Monoclonal antibody (66805-1-Ig)

    WB analysis of human heart using 66805-1-Ig (same clone as 66805-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 66805-1-Ig (AQP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66805-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human skeletal muscle tissue using AQP1 Monoclonal antibody (66805-1-Ig)

    WB analysis of human skeletal muscle using 66805-1-Ig (same clone as 66805-1-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66805-1-Ig (AQP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66805-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig kidney tissue using AQP1 Monoclonal antibody (66805-1-Ig)

    WB analysis of pig kidney using 66805-1-Ig (same clone as 66805-1-PBS)

    pig kidney tissue were subjected to SDS PAGE followed by western blot with 66805-1-Ig (AQP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66805-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using AQP1 Monoclonal antibody (66805-1-Ig)

    IHC staining of human kidney using 66805-1-Ig (same clone as 66805-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66805-1-Ig (AQP1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66805-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using AQP1 Monoclonal antibody (66805-1-Ig)

    IHC staining of human kidney using 66805-1-Ig (same clone as 66805-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66805-1-Ig (AQP1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66805-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human kidney tissue using AQP1 Monoclonal antibody (66805-1-Ig)

    IF Staining of human kidney using 66805-1-Ig (same clone as 66805-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using AQP1 antibody (66805-1-Ig, Clone: 2E5D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66805-1-PBS in a different storage buffer formulation.

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AQP1 Monoclonal antibody, PBS Only

AQP1 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 66805-1-PBS
Clone No.2E5D6

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

AQP 1, AQP CHIP, AQP1, Aquaporin 1, Aquaporin CHIP, CHIP28, CO, Urine water channel

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66805-1-PBS targets AQP1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, pig samples.

Tested Reactivity Human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AQP1 fusion protein Ag14093 相同性解析による交差性が予測される生物種
Full Name aquaporin 1 (Colton blood group)
Calculated molecular weight 269 aa, 29 kDa
Observed molecular weight 28 kDa, 38-40 kDa
GenBank accession numberBC022486
Gene Symbol AQP1
Gene ID (NCBI) 358
RRIDAB_2882148
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP29972
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

AQP1 is a member of aquaporins (AQPs) that are small membrane-spanning proteins facilitating water transport. AQP1 is expressed in most tissues in the mammalian body. Alterations of AQP1 expression have been linked to variety of diseases, including cancer. The predicted molecular weight of AQP1 is around 28 kDa, while highly glycosylated form can also be observed around 40-45 kDa. (1530176)