Validation Data Gallery
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Tested Applications
| Positive WB detected in | human heart tissue, human skeletal muscle tissue, pig kidney tissue |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:20000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IF | See 3 publications below |
Product Information
66805-1-Ig targets AQP1 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, pig samples.
| Tested Reactivity | Human, pig |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | AQP1 fusion protein Ag14093 相同性解析による交差性が予測される生物種 |
| Full Name | aquaporin 1 (Colton blood group) |
| Calculated molecular weight | 269 aa, 29 kDa |
| Observed molecular weight | 28 kDa, 38-40 kDa |
| GenBank accession number | BC022486 |
| Gene Symbol | AQP1 |
| Gene ID (NCBI) | 358 |
| RRID | AB_2882148 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P29972 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
AQP1 is a member of aquaporins (AQPs) that are small membrane-spanning proteins facilitating water transport. AQP1 is expressed in most tissues in the mammalian body. Alterations of AQP1 expression have been linked to variety of diseases, including cancer. The predicted molecular weight of AQP1 is around 28 kDa, while highly glycosylated form can also be observed around 40-45 kDa. (1530176)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for AQP1 antibody 66805-1-Ig | Download protocol |
| IHC protocol for AQP1 antibody 66805-1-Ig | Download protocol |
| IF protocol for AQP1 antibody 66805-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
iScience Mutational burden of XPNPEP3 leads to defects in mitochondrial complex I and cilia in NPHPL1 | ||
Adv Sci (Weinh) Behind the Indolent Facade: Uncovering the Molecular Features and Malignancy Potential in Lung Minimally Invasive Adenocarcinoma by Single-Cell Transcriptomics | ||
Clin Transl Med Single-cell RNA sequencing of pediatric renal tissues revealed the potential relationship between immunoglobulin A nephropathy and immunoglobulin A vasculitis with nephritis |