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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated AQP1 Monoclonal antibody

AQP1 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2E5D6

Cat no : CL488-66805

Synonyms

AQP 1, AQP CHIP, AQP1, Aquaporin 1, Aquaporin CHIP, CHIP28, CO, Urine water channel

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human kidney tissue using CoraLite® Plus 488-conjugated AQP1 Monoclonal anti (CL488-66805)

    IF Staining of human kidney using CL488-66805

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using CoraLite® Plus 488 AQP1 antibody (CL488-66805, Clone: 2E5D6 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of human kidney tissue using CoraLite® Plus 488-conjugated AQP1 Monoclonal anti (CL488-66805)

    IF Staining of human kidney using CL488-66805

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using CoraLite® Plus 488 AQP1 antibody (CL488-66805, Clone: 2E5D6 ) at dilution of 1:400, CoraLite®594 Podocalyxin antibody (CL594-18150, red).

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Tested Applications

Positive IF-P detected inhuman kidney tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66805 targets AQP1 in IF-P applications and shows reactivity with Human, pig samples.

Tested Reactivity Human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AQP1 fusion protein Ag14093 相同性解析による交差性が予測される生物種
Full Name aquaporin 1 (Colton blood group)
Calculated molecular weight 269 aa, 29 kDa
Observed molecular weight 28 kDa, 38-40 kDa
GenBank accession numberBC022486
Gene symbol AQP1
Gene ID (NCBI) 358
RRIDAB_2919375
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AQP1 is a member of aquaporins (AQPs) that are small membrane-spanning proteins facilitating water transport. AQP1 is expressed in most tissues in the mammalian body. Alterations of AQP1 expression have been linked to variety of diseases, including cancer. The predicted molecular weight of AQP1 is around 28 kDa, while highly glycosylated form can also be observed around 40-45 kDa. (1530176)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 AQP1 antibody CL488-66805Download protocol
Standard Protocols
Click here to view our Standard Protocols