Validation Data Gallery
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Tested Applications
| Positive IF/ICC detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-66550 targets ARC in IF/ICC applications and shows reactivity with Human, Mouse, Rat, Pig, rabbit samples.
| Tested Reactivity | Human, Mouse, Rat, Pig, rabbit |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | ARC fusion protein Ag9445 相同性解析による交差性が予測される生物種 |
| Full Name | activity-regulated cytoskeleton-associated protein |
| Calculated molecular weight | 396 aa, 45 kDa |
| Observed molecular weight | 50 kDa |
| GenBank accession number | BC012321 |
| Gene Symbol | ARC |
| Gene ID (NCBI) | 23237 |
| RRID | AB_2934478 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q7LC44 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ARC, also named as KIAA0278 and Arg3.1, mediates endocytosis of neuronal AMPA-type glutamate receptors (AMPARs). It is required for consolidation of synaptic plasticity as well as formation of long-term memory. ARC plays a role in the regulation of cell morphology and cytoskeletal organization. It is required in the stress fiber dynamics and cell migration. Recent study shows that expression of Arc can regulate cognitive flexibility.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 ARC antibody CL488-66550 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |