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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ARF1 Monoclonal antibody (68069-1-Ig)

    WB analysis using 68069-1-Ig (same clone as 68069-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68069-1-Ig (ARF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68069-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ARF1 Monoclonal antibody (68069-1-Ig)

    IF Staining of HeLa using 68069-1-Ig (same clone as 68069-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ARF1 antibody (68069-1-Ig, Clone: 1C10E3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68069-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ARF1 Monoclonal antibody (68069-1-Ig)

    IF Staining of HeLa using 68069-1-Ig (same clone as 68069-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ARF1 antibody (68069-1-Ig, Clone: 1C10E3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 68069-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ARF1 Monoclonal antibody (68069-1-Ig)

    IF Staining of HeLa using 68069-1-Ig (same clone as 68069-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ARF1 antibody (68069-1-Ig, Clone: 1C10E3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68069-1-PBS in a different storage buffer formulation.

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ARF1 Monoclonal antibody, PBS Only

ARF1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68069-1-PBS
Clone No.1C10E3

Host / Isotype

Mouse / IgG1

Reactivity

rabbit, pig, rat, mouse, human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

EC:3.6.5.2, ADP-ribosylation factor 1, ADP ribosylation factor 1, 1C10E3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68069-1-PBS targets ARF1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with rabbit, pig, rat, mouse, human samples.

Tested Reactivity rabbit, pig, rat, mouse, human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ARF1 fusion protein Ag28021 相同性解析による交差性が予測される生物種
Full Name ADP-ribosylation factor 1
Calculated molecular weight 21 kDa
Observed molecular weight18-21 kDa
GenBank accession numberBC009247
Gene Symbol ARF1
Gene ID (NCBI) 375
RRIDAB_2918810
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP84077
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. It mainly functions in coat recruitment.(PMID: 7759471, PMID: 16042562).