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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ARF1 Monoclonal antibody

ARF1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

1C10E3

Cat no : 68069-1-Ig

Synonyms

EC:3.6.5.2, ADP-ribosylation factor 1, ADP ribosylation factor 1, 1C10E3

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using ARF1 Monoclonal antibody (68069-1-Ig)

    WB analysis using 68069-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68069-1-Ig (ARF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ARF1 Monoclonal antibody (68069-1-Ig)

    IF Staining of HeLa using 68069-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ARF1 antibody (68069-1-Ig, Clone: 1C10E3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ARF1 Monoclonal antibody (68069-1-Ig)

    IF Staining of HeLa using 68069-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ARF1 antibody (68069-1-Ig, Clone: 1C10E3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ARF1 Monoclonal antibody (68069-1-Ig)

    IF Staining of HeLa using 68069-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ARF1 antibody (68069-1-Ig, Clone: 1C10E3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inA431 cells, LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68069-1-Ig targets ARF1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ARF1 fusion protein Ag28021 相同性解析による交差性が予測される生物種
Full Name ADP-ribosylation factor 1
Calculated molecular weight 21 kDa
Observed molecular weight18-21 kDa
GenBank accession numberBC009247
Gene symbol ARF1
Gene ID (NCBI) 375
RRIDAB_2918810
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. It mainly functions in coat recruitment.(PMID: 7759471, PMID: 16042562).

Protocols

Product Specific Protocols
WB protocol for ARF1 antibody 68069-1-IgDownload protocol
IF protocol for ARF1 antibody 68069-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

Cell Metab

Astrocytic LRP1 enables mitochondria transfer to neurons and mitigates brain ischemic stroke by suppressing ARF1 lactylation

Authors - Jian Zhou
View Article