Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-68069 targets ARF1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, rabbit samples.
| Tested Reactivity | human, mouse, rat, rabbit |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | ARF1 fusion protein Ag28021 相同性解析による交差性が予測される生物種 |
| Full Name | ADP-ribosylation factor 1 |
| Calculated molecular weight | 21 kDa |
| Observed molecular weight | 18-21 kDa |
| GenBank accession number | BC009247 |
| Gene Symbol | ARF1 |
| Gene ID (NCBI) | 375 |
| RRID | AB_2919562 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P84077 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. It mainly functions in coat recruitment.(PMID: 7759471, PMID: 16042562).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 ARF1 antibody CL488-68069 | Download protocol |
| FC protocol for CL Plus 488 ARF1 antibody CL488-68069 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |