CoraLite® Plus 488-conjugated Arginase-1 Polyclonal antibody
Arginase-1 Polyclonal Antibody for IF-P
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF-P
Conjugate
CoraLite® Plus 488 Fluorescent Dye
Cat no : CL488-16001
Synonyms
Validation Data Gallery
Tested Applications
Positive IF-P detected in | human liver cancer tissue |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL488-16001 targets Arginase-1 in IF-P applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Arginase-1 fusion protein Ag8595 相同性解析による交差性が予測される生物種 |
Full Name | arginase, liver |
Calculated molecular weight | 236aa,25 kDa; 322aa,35 kDa |
Observed molecular weight | 35-36 kDa |
GenBank accession number | BC005321 |
Gene symbol | Arginase-1 |
Gene ID (NCBI) | 383 |
RRID | AB_2919127 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Arginase-1 (Liver arginase) belongs to the arginase family. ARG1 is a novel immunohistochemical marker of hepatocellular differentiation in fine needle aspiration cytology and a marker of hepatocytes and hepatocellular neoplasms. ARG1 is closely associated with alternative macrophage activation(PMID:12098359) and ARG1 has been shown to protect motor neurons from trophic factor deprivation and allow sensory neurons to overcome neurite outgrowth inhibition by myelin proteins(PMID: 20071539). It can exist as a homotrimer(PMID:16141327) and it has 3 isoforms produced by alternative splicing. Defects in ARG1 are the cause of argininemia (ARGIN). Deletion or TNF-mediated restriction of ARG1 unleashes the production of NO by NOS2, which is critical for pathogen control.(PMID:27117406). ARG1 has 3 isoforms with the molecular mass of 25, 35 and 36 kDa.
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 Arginase-1 antibody CL488-16001 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |