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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Arginase-1 Polyclonal antibody

Arginase-1 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-16001

Synonyms

ARG1, Arginase 1, arginase, liver, Liver type arginase, Type I arginase

Validation Data Gallery

Applications:
  • IF Staining of human liver cancer using CL488-16001

    IF Staining of human liver cancer using CL488-16001

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite® Plus 488 ARG1 antibody (CL488-16001) at dilution of 1:400.

Tested Applications

Positive IF detected inhuman liver cancer tissue
Planning an IHC experiment? We recommend our IHCeasy ARG1 Ready-To-Use IHC Kit. ARG1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 16001-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

CL488-16001 targets Arginase-1 in IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Arginase-1 fusion protein Ag8595 相同性解析による交差性が予測される生物種
Full Name arginase, liver
Calculated molecular weight236aa,25 kDa; 322aa,35 kDa
Observed molecular weight 35-36 kDa
GenBank accession numberBC005321
Gene symbol ARG1
Gene ID (NCBI) 383
RRIDAB_2919127
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Arginase-1 (Liver arginase) belongs to the arginase family. ARG1 is a novel immunohistochemical marker of hepatocellular differentiation in fine needle aspiration cytology and a marker of hepatocytes and hepatocellular neoplasms. ARG1 is closely associated with alternative macrophage activation(PMID:12098359) and ARG1 has been shown to protect motor neurons from trophic factor deprivation and allow sensory neurons to overcome neurite outgrowth inhibition by myelin proteins(PMID: 20071539). It can exist as a homotrimer(PMID:16141327) and it has 3 isoforms produced by alternative splicing. Defects in ARG1 are the cause of argininemia (ARGIN). Deletion or TNF-mediated restriction of ARG1 unleashes the production of NO by NOS2, which is critical for pathogen control.(PMID:27117406). ARG1 has 3 isoforms with the molecular mass of 25, 35 and 36 kDa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Arginase-1 antibody CL488-16001Download protocol
Standard Protocols
Click here to view our Standard Protocols