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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ARK5 Polyclonal antibody (22723-1-AP)

    WB analysis using 22723-1-AP (same clone as 22723-1-PBS)

    rat heart tissue were subjected to SDS PAGE followed by western blot with 22723-1-AP (ARK5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22723-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using ARK5 Polyclonal antibody (22723-1-AP)

    WB analysis of mouse brain using 22723-1-AP (same clone as 22723-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22723-1-AP (ARK5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22723-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ARK5 Polyclonal antibody (22723-1-AP)

    IHC staining of human colon cancer using 22723-1-AP (same clone as 22723-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22723-1-AP (ARK5 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22723-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ARK5 Polyclonal antibody (22723-1-AP)

    IHC staining of human colon cancer using 22723-1-AP (same clone as 22723-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22723-1-AP (ARK5 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22723-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using ARK5 Polyclonal antibody (22723-1-AP)

    IHC staining of human heart using 22723-1-AP (same clone as 22723-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22723-1-AP (ARK5 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22723-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using ARK5 Polyclonal antibody (22723-1-AP)

    IHC staining of human heart using 22723-1-AP (same clone as 22723-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22723-1-AP (ARK5 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22723-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

ARK5 Polyclonal antibody, PBS Only

ARK5 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 22723-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22723-1-PBS targets ARK5 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARK5 fusion protein Ag18612 相同性解析による交差性が予測される生物種
Full Name NUAK family, SNF1-like kinase, 1
Calculated molecular weight 661 aa, 74 kDa
Observed molecular weight 74 kDa
GenBank accession numberBC152462
Gene Symbol ARK5
Gene ID (NCBI) 9891
RRIDAB_2879157
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDO60285
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

NUAK1 is a member of the human adenosine monophosphate (AMP)-activated protein kinase family that has been identified as a key tumor cell survival factor. It is also named as ARK5, KIAA0537, OMPHK1 and belongs to the protein kinase superfamily. The physiological role of NUAK1 is potentially to suppress glucose uptake through negative regulation of INS signaling in oxidative muscle. This protein has 2 isoforms produced by alternative splicing.