Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells, K-562 cells, L02 cells |
| Positive IP detected in | L02 cells |
| Positive IF/ICC detected in | MDCK cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
30332-1-AP targets ARL13B in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Guinea Pig / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ARL13B fusion protein Ag12015 相同性解析による交差性が予測される生物種 |
| Full Name | ADP-ribosylation factor-like 13B |
| Calculated molecular weight | 48 kDa |
| Observed molecular weight | 48-55 kDa |
| GenBank accession number | BC094725 |
| Gene Symbol | ARL13B |
| Gene ID (NCBI) | 200894 |
| RRID | AB_3086296 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q3SXY8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870).This antibody detects two specific bands at 60 kDa and 48 kDa. Arl13b is predicted to be a 48 kDa protein, and the 60 kDa band is likely to represent a modified form of Arl13b. ARL13B can be used to mark the cilia (PMID:22072986). The second antibody is used against guinea pigs.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ARL13B antibody 30332-1-AP | Download protocol |
| IF protocol for ARL13B antibody 30332-1-AP | Download protocol |
| IP protocol for ARL13B antibody 30332-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |