CoraLite® Plus 488-conjugated ARL13B Polyclonal antibody
ARL13B Polyclonal Antibody for IF/ICC
Host / Isotype
Guinea Pig / IgG
Reactivity
Human
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
Cat no : CL488-30332
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | MDCK cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-30332 targets ARL13B in IF/ICC applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Guinea Pig / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ARL13B fusion protein Ag12015 相同性解析による交差性が予測される生物種 |
| Full Name | ADP-ribosylation factor-like 13B |
| Calculated molecular weight | 48 kDa |
| Observed molecular weight | 48-55 kDa |
| GenBank accession number | BC094725 |
| Gene symbol | ARL13B |
| Gene ID (NCBI) | 200894 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870).This antibody detects two specific bands at 60 kDa and 48 kDa. Arl13b is predicted to be a 48 kDa protein, and the 60 kDa band is likely to represent a modified form of Arl13b. ARL13B can be used to mark the cilia (PMID:22072986). The second antibody is used against guinea pigs.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 ARL13B antibody CL488-30332 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
