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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated ARL13B Polyclonal antibody

ARL13B Polyclonal Antibody for IF/ICC

Host / Isotype

Guinea Pig / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-30332

Synonyms

ARL2-like protein 1, ARL2L1, ARL2 like protein 1, ADP-ribosylation factor-like protein 2-like 1, ADP-ribosylation factor-like protein 13B

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using CoraLite® Plus 488-conjugated ARL13B Polyclonal an (CL488-30332)

    IF Staining of hTERT-RPE1 using CL488-30332

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CoraLite® Plus 488 ARL13B antibody (CL488-30332) at dilution of 1:200, CoraLite®594 Acetyl-Tubulin (Lys40) antibody (CL594-66200, Clone: 7E5H8, red).

  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using CoraLite® Plus 488-conjugated ARL13B Polyclonal an (CL488-30332)

    IF Staining of MDCK using CL488-30332

    Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CoraLite® Plus 488 ARL13B antibody (CL488-30332) at dilution of 1:200, CoraLite®594 ARL13B antibody (CL594-17711, red).

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Tested Applications

Positive IF/ICC detected inhTERT-RPE1 cells, MDCK cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-30332 targets ARL13B in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Guinea Pig / IgG
Class Polyclonal
Type Antibody
Immunogen ARL13B fusion protein Ag12015 相同性解析による交差性が予測される生物種
Full Name ADP-ribosylation factor-like 13B
Calculated molecular weight 48 kDa
Observed molecular weight 48-55 kDa
GenBank accession numberBC094725
Gene symbol ARL13B
Gene ID (NCBI) 200894
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870).This antibody detects two specific bands at 60 kDa and 48 kDa. Arl13b is predicted to be a 48 kDa protein, and the 60 kDa band is likely to represent a modified form of Arl13b. ARL13B can be used to mark the cilia (PMID:22072986). The second antibody is used against guinea pigs.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 ARL13B antibody CL488-30332Download protocol
Standard Protocols
Click here to view our Standard Protocols