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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ARL13B Recombinant antibody

ARL13B Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3H5

Cat no : 82834-2-RR

Synonyms

ARL2-like protein 1, ARL2L1, ARL2 like protein 1, ADP-ribosylation factor-like protein 2-like 1, ADP-ribosylation factor-like protein 13B

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using ARL13B Recombinant antibody (82834-2-RR)

    IF Staining of hTERT-RPE1 using 82834-2-RR

    Immunofluorescent analysis of (4% PFA) fixed Starvation treated hTERT-RPE1 cells using ARL13B antibody (82834-2-RR, Clone: 3H5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Acetyl-Tubulin (Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using ARL13B Recombinant antibody (82834-2-RR)

    IF Staining of hTERT-RPE1 using 82834-2-RR

    Immunofluorescent analysis of (4% PFA) fixed Starvation treated hTERT-RPE1 cells using ARL13B antibody (82834-2-RR, Clone: 3H5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Acetyl-Tubulin (Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

  • Affinity and Kinetic Characterization of 82834-2-RR

    Affinity and Kinetic Characterization of 82834-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82834-2-RR against Human ARL13B were performed. The affinity constant is 1.25 nM.

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Tested Applications

Positive IF/ICC detected inStarvation treated hTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82834-2-RR targets ARL13B in IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ARL13B fusion protein Ag12015 相同性解析による交差性が予測される生物種
Full Name ADP-ribosylation factor-like 13B
Calculated molecular weight 48 kDa
GenBank accession numberBC094725
Gene symbol ARL13B
Gene ID (NCBI) 200894
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870). ARL13B can be used to mark the cilia (PMID:22072986). The second antibody is used against guinea pigs.

Protocols

Product Specific Protocols
IF protocol for ARL13B antibody 82834-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols